3ALR

Crystal structure of Nanos

3ALR の概要

• エントリーDOI: 10.2210/pdb3alr/pdb
• 分子名称: Nanos protein, ZINC ION (3 entities in total)
• 機能のキーワード: zinc-finger, translational repression, rna, 3'-utr, metal binding protein
• 由来する生物種: Danio rerio (Zebrafish)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 47290.79

構造登録者

Hashimoto, H.,Hara, K.,Hishiki, A.,Kawaguchi, S.,Shichijo, N.,Nakamura, K.,Unzai, S.,Tamaru, Y.,Shimizu, T.,Sato, M. (登録日: 2010-08-06, 公開日: 2011-02-02, 最終更新日: 2024-03-13)

主引用文献

Hashimoto, H.,Hara, K.,Hishiki, A.,Kawaguchi, S.,Shichijo, N.,Nakamura, K.,Unzai, S.,Tamaru, Y.,Shimizu, T.,Sato, M.
Crystal structure of zinc-finger domain of Nanos and its functional implications
Embo Rep., 11:848-853, 2010

PubMed Abstract: Nanos is an RNA-binding protein that is involved in the development and maintenance of germ cells. In combination with Pumilio, Nanos binds to the 3' untranslated region of a messenger RNA and represses its translation. Nanos has two conserved Cys-Cys-His-Cys zinc-finger motifs that are indispensable for its function. In this study, we have determined the crystal structure of the zinc-finger domain of zebrafish Nanos, for the first time revealing that Nanos adopts a novel zinc-finger structure. In addition, Nanos has a conserved basic surface that is directly involved in RNA binding. Our results provide the structural basis for further studies to clarify Nanos function.

PubMed: 20948543
DOI: 10.1038/embor.2010.155

実験手法

X-RAY DIFFRACTION (2.1 Å)