3AKK

Crystal structure of A Helicobacter pylori proinflammatory kinase CtkA

Summary for 3AKK

• Entry DOI: 10.2210/pdb3akk/pdb
• Related: 3AKJ 3AKL
• Descriptor: CtkA, MAGNESIUM ION, ADENOSINE-5'-DIPHOSPHATE, ... (4 entities in total)
• Functional Keywords: protein kinase, transferase
• Biological source: Helicobacter pylori (Campylobacter pylori)
• Total number of polymer chains: 4
• Total formula weight: 152420.78

Authors

Kim, D.J.,Suh, S.W. (deposition date: 2010-07-14, release date: 2010-12-01, Last modification date: 2024-03-13)

Primary citation

Kim, D.J.,Park, K.-S.,Kim, J.-H.,Yang, S.-H.,Yoon, J.Y.,Han, B.-G.,Kim, H.S.,Lee, S.J.,Jang, J.Y.,Kim, K.H.,Kim, M.J.,Song, J.-S.,Kim, H.-J.,Park, C.-M.,Lee, S.-K.,Lee, B.I.,Suh, S.W.
Helicobacter pylori proinflammatory protein up-regulates NF-kappaB as a cell-translocating Ser/Thr kinase
Proc.Natl.Acad.Sci.USA, 107:21418-21423, 2010

PubMed Abstract: There has been considerable interest in virulence genes in the plasticity region of Helicobacter pylori, but little is known about many of these genes. JHP940, one of the virulence factors encoded by the plasticity region of H. pylori strain J99, is a proinflammatory protein that induces tumor necrosis factor-alpha and interleukin-8 secretion as well as enhanced translocation of NF-κB in cultured macrophages. Here we have characterized the structure and function of JHP940 to provide the framework for better understanding its role in inflammation by H. pylori. Our work demonstrates that JHP940 is the first example of a eukaryotic-type Ser/Thr kinase from H. pylori. We show that JHP940 is catalytically active as a protein kinase and translocates into cultured human cells. Furthermore, the kinase activity is indispensable for indirectly up-regulating phosphorylation of NF-κB p65 at Ser276. Our results, taken together, contribute significantly to understanding the molecular basis of the role of JHP940 in inflammation and subsequent pathogenesis caused by H. pylori. We propose to rename the jhp940 gene as ctkA (cell translocating kinase A).

PubMed: 21098302
DOI: 10.1073/pnas.1010153107

Experimental method

X-RAY DIFFRACTION (2.5 Å)