3AJJ
Crystal Structure of d(CGCGGATf5UCGCG): 5-Formyluridine/Guanosine Base-pair in B-DNA
Summary for 3AJJ
| Entry DOI | 10.2210/pdb3ajj/pdb |
| Related | 3AJK 3AJL |
| Descriptor | 5'-D(*CP*GP*CP*GP*GP*AP*TP*(UFR)P*CP*GP*CP*G)-3' (2 entities in total) |
| Functional Keywords | modified nucleotide, formyluridine, dna damage, b-dna, double helix, deoxyribonucleic acid, dna |
| Total number of polymer chains | 2 |
| Total formula weight | 7386.75 |
| Authors | Tsunoda, M.,Sakaue, T.,Ueno, Y.,Matsuda, A.,Takenaka, A. (deposition date: 2010-06-07, release date: 2011-04-27, Last modification date: 2023-11-01) |
| Primary citation | Tsunoda, M.,Sakaue, T.,Naito, S.,Sunami, T.,Abe, N.,Ueno, Y.,Matsuda, A.,Takenaka, A. Insights into the structures of DNA damaged by hydroxyl radical: crystal structures of DNA duplexes containing 5-formyluracil J Nucleic Acids, 2010:107289-107289, 2010 Cited by PubMed Abstract: Hydroxyl radicals are potent mutagens that attack DNA to form various base and ribose derivatives. One of the major damaged thymine derivatives is 5-formyluracil (fU), which induces pyrimidine transition during replication. In order to establish the structural basis for such mutagenesis, the crystal structures of two kinds of DNA d(CGCGRATfUCGCG) with R = A/G have been determined by X-ray crystallography. The fU residues form a Watson-Crick-type pair with A and two types of pairs (wobble and reversed wobble) with G, the latter being a new type of base pair between ionized thymine base and guanine base. In silico structural modeling suggests that the DNA polymerase can accept the reversed wobble pair with G, as well as the Watson-Crick pair with A. PubMed: 20976303DOI: 10.4061/2010/107289 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.02 Å) |
Structure validation
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