3ADY

Crystal structure of DotD from Legionella

3ADY の概要

• エントリーDOI: 10.2210/pdb3ady/pdb
• 分子名称: DotD (2 entities in total)
• 機能のキーワード: 3-layer(bab) sandwich, mth1598-like, proton transport
• 由来する生物種: Legionella pneumophila
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16191.57

構造登録者

Imada, K.,Nakano, N.,Kubori, T.,Kinoshita, M.,Nagai, H. (登録日: 2010-01-29, 公開日: 2010-11-03, 最終更新日: 2024-03-13)

主引用文献

Nakano, N.,Kubori, T.,Kinoshita, M.,Imada, K.,Nagai, H.
Crystal structure of Legionella DotD: insights into the relationship between type IVB and type II/III secretion systems
Plos Pathog., 6:e1001129-e1001129, 2010

PubMed Abstract: The Dot/Icm type IVB secretion system (T4BSS) is a pivotal determinant of Legionella pneumophila pathogenesis. L. pneumophila translocate more than 100 effector proteins into host cytoplasm using Dot/Icm T4BSS, modulating host cellular functions to establish a replicative niche within host cells. The T4BSS core complex spanning the inner and outer membranes is thought to be made up of at least five proteins: DotC, DotD, DotF, DotG and DotH. DotH is the outer membrane protein; its targeting depends on lipoproteins DotC and DotD. However, the core complex structure and assembly mechanism are still unknown. Here, we report the crystal structure of DotD at 2.0 Å resolution. The structure of DotD is distinct from that of VirB7, the outer membrane lipoprotein of the type IVA secretion system. In contrast, the C-terminal domain of DotD is remarkably similar to the N-terminal subdomain of secretins, the integral outer membrane proteins that form substrate conduits for the type II and the type III secretion systems (T2SS and T3SS). A short β-segment in the otherwise disordered N-terminal region, located on the hydrophobic cleft of the C-terminal domain, is essential for outer membrane targeting of DotH and Dot/Icm T4BSS core complex formation. These findings uncover an intriguing link between T4BSS and T2SS/T3SS.

PubMed: 20949065
DOI: 10.1371/journal.ppat.1001129

実験手法

X-RAY DIFFRACTION (2 Å)