3AAC

Small heat shock protein hsp14.0 with the mutations of I120F and I122F in the form II crystal

Summary for 3AAC

• Entry DOI: 10.2210/pdb3aac/pdb
• Related: 3AAB
• Descriptor: Putative uncharacterized protein ST1653 (2 entities in total)
• Functional Keywords: alpha-crystallin domain, chaperone
• Biological source: Sulfolobus tokodaii
• Total number of polymer chains: 2
• Total formula weight: 28264.52

Authors

Takeda, K.,Hayashi, T.,Abe, T.,Hirano, Y.,Hanazono, Y.,Yohda, M.,Miki, K. (deposition date: 2009-11-13, release date: 2010-11-17, Last modification date: 2024-03-13)

Primary citation

Takeda, K.,Hayashi, T.,Abe, T.,Hirano, Y.,Hanazono, Y.,Yohda, M.,Miki, K.
Dimer structure and conformational variability in the N-terminal region of an archaeal small heat shock protein, StHsp14.0
J.Struct.Biol., 174:92-99, 2011

PubMed Abstract: Small heat shock proteins (sHsps), which are categorized into a class of molecular chaperones, bind and stabilize denatured proteins to prevent aggregation. The sHsps undergo transition between different oligomeric states to control their hydrophobicity. So far, only the structures of sHsps in large oligomeric states have been reported. Here we report the structure of StHsp14.0 from Sulfolobus tokodaii in the dimeric state, which is formed by means of a mutation at the C-terminal IXI/V motif. The dimer is the sole building block in two crystal forms, and the dimeric mode is the same as that in the large oligomers. The N-terminal helix has variety in its conformation. Furthermore, spectroscopic and biochemical experiments were performed to investigate the conformational variability at the N-terminus. The structural, dynamical and oligomeric properties suggest that chaperone activity of StHsp14.0 is mediated by partially dissolved oligomers.

PubMed: 21195185
DOI: 10.1016/j.jsb.2010.12.006

Experimental method

X-RAY DIFFRACTION (2.4 Å)