3A79

Crystal structure of TLR2-TLR6-Pam2CSK4 complex

3A79 の概要

• エントリーDOI: 10.2210/pdb3a79/pdb
• 関連するPDBエントリー: 3A7B 3A7C
• 分子名称: Toll-like receptor 2, Variable lymphocyte receptor B, Toll-like receptor 6, Variable lymphocyte receptor B, Pam2CSK4, ... (7 entities in total)
• 機能のキーワード: toll-like receptor, diacyl lipopeptide, innate immunity, leucine rich repeat, cell membrane, cytoplasmic vesicle, disulfide bond, glycoprotein, immune response, inflammatory response, leucine-rich repeat, membrane, receptor, transmembrane, phosphoprotein, immune system
• 由来する生物種: Mus musculus (mouse, Inshore hagfish); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 134737.60

構造登録者

Kang, J.Y.,Jin, M.S.,Lee, J.-O. (登録日: 2009-09-20, 公開日: 2009-11-24, 最終更新日: 2024-10-30)

主引用文献

Kang, J.Y.,Nan, X.,Jin, M.S.,Youn, S.-J.,Ryu, Y.H.,Mah, S.,Han, S.H.,Lee, H.,Paik, S.-G.,Lee, J.-O.
Recognition of lipopeptide patterns by Toll-like receptor 2-Toll-like receptor 6 heterodimer
Immunity, 31:873-884, 2009

PubMed Abstract: Toll-like receptor 2 (TLR2) initiates potent immune responses by recognizing diacylated and triacylated lipopeptides. Its ligand specificity is controlled by whether it heterodimerizes with TLR1 or TLR6. We have determined the crystal structures of TLR2-TLR6-diacylated lipopeptide, TLR2-lipoteichoic acid, and TLR2-PE-DTPA complexes. PE-DTPA, 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine-N-diethylenetriaminepentaacetic acid, is a synthetic phospholipid derivative. Two major factors contribute to the ligand specificity of TLR2-TLR1 or TLR2-TLR6 heterodimers. First, the lipid channel of TLR6 is blocked by two phenylalanines. Simultaneous mutation of these phenylalanines made TLR2-TLR6 fully responsive not only to diacylated but also to triacylated lipopeptides. Second, the hydrophobic dimerization interface of TLR2-TLR6 is increased by 80%, which compensates for the lack of amide lipid interaction between the lipopeptide and TLR2-TLR6. The structures of the TLR2-lipoteichoic acid and the TLR2-PE-DTPA complexes demonstrate that a precise interaction pattern of the head group is essential for a robust immune response by TLR2 heterodimers.

PubMed: 19931471
DOI: 10.1016/j.immuni.2009.09.018

実験手法

X-RAY DIFFRACTION (2.9 Å)