3A5T

Crystal structure of MafG-DNA complex

3A5T の概要

• エントリーDOI: 10.2210/pdb3a5t/pdb
• 分子名称: Transcription factor MafG, 5'-D(*CP*TP*GP*AP*TP*GP*AP*GP*TP*CP*AP*GP*CP*AP*C)-3', 5'-D(*GP*TP*GP*CP*TP*GP*AP*CP*TP*CP*AP*TP*CP*AP*G)-3', ... (5 entities in total)
• 機能のキーワード: protein-dna complex, bzip factor, acetylation, dna-binding, isopeptide bond, nucleus, repressor, transcription, transcription regulation, ubl conjugation, transcription regulator-dna complex, transcription regulator/dna
• 由来する生物種: Mus musculus (mouse); 詳細
• 細胞内の位置: Nucleus : O54790
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 34246.98

構造登録者

Kurokawa, H.,Motohashi, H.,Sueno, S.,Kimura, M.,Takagawa, H.,Kanno, Y.,Yamamoto, M.,Tanaka, T. (登録日: 2009-08-11, 公開日: 2009-10-13, 最終更新日: 2024-10-23)

主引用文献

Kurokawa, H.,Motohashi, H.,Sueno, S.,Kimura, M.,Takagawa, H.,Kanno, Y.,Yamamoto, M.,Tanaka, T.
Structural Basis of Alternative DNA Recognition by Maf Transcription Factors
Mol.Cell.Biol., 29:6232-6244, 2009

PubMed Abstract: Maf transcription factors constitute a family of the basic region-leucine zipper (bZip) factors and recognize unusually long DNA motifs (13 or 14 bp), termed the Maf recognition element (MARE). The MARE harbors extended GC sequences on each side of its core motif, which is similar to TRE or CRE (7 or 8 bp) recognized by the AP1 and CREB/ATF families, respectively. To ascertain the structural basis governing the acquirement of such unique DNA recognition, we determined the crystal structure of the MafG-DNA complex. Each MafG monomer consists of three helices in which the carboxyl-terminal long helix organizes one DNA-contacting element and one coiled-coil dimer formation element. To our surprise, two well-conserved residues, Arg57 and Asn61 in the basic region, play critical roles in Maf-specific DNA recognition. These two residues show unique side-chain orientations and interact directly with the extended GC bases. Maf-specific residues in the amino-terminal and basic regions appear to indirectly stabilize MARE recognition through DNA backbone phosphate interactions. This study revealed an alternative DNA recognition mechanism of the bZip factors that bestows specific target gene profiles upon Maf homodimers or Maf-containing heterodimers.

PubMed: 19797082
DOI: 10.1128/MCB.00708-09

実験手法

X-RAY DIFFRACTION (2.8 Å)