3A50
Structure of cytochrome P450 Vdh mutant (Vdh-K1) obtained by directed evolution with bound vitamin D3
Summary for 3A50
Entry DOI | 10.2210/pdb3a50/pdb |
Related | 3A4G 3A4H 3A4Z 3A51 |
Descriptor | Vitamin D hydroxylase, PROTOPORPHYRIN IX CONTAINING FE, (1S,3Z)-3-[(2E)-2-[(1R,3AR,7AS)-7A-METHYL-1-[(2R)-6-METHYLHEPTAN-2-YL]-2,3,3A,5,6,7-HEXAHYDRO-1H-INDEN-4-YLIDENE]ETHYLI DENE]-4-METHYLIDENE-CYCLOHEXAN-1-OL, ... (7 entities in total) |
Functional Keywords | cytochrome p450, vitamin d3 hydroxylase, hemoprotein, monooxygenase, directed evolution, oxidoreductase |
Biological source | Pseudonocardia autotrophica |
Cellular location | Cytoplasm : C4B644 |
Total number of polymer chains | 5 |
Total formula weight | 233723.98 |
Authors | Yasutake, Y.,Fujii, Y.,Cheon, W.K.,Arisawa, A.,Tamura, T. (deposition date: 2009-07-24, release date: 2010-07-28, Last modification date: 2023-11-01) |
Primary citation | Yasutake, Y.,Fujii, Y.,Nishioka, T.,Cheon, W.K.,Arisawa, A.,Tamura, T. Structural evidence for enhancement of sequential vitamin D3 hydroxylation activities by directed evolution of cytochrome P450 vitamin D3 hydroxylase J.Biol.Chem., 285:31193-31201, 2010 Cited by PubMed: 20667833DOI: 10.1074/jbc.M110.147009 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2.05 Å) |
Structure validation
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