3WG1
Crystal structure of Agrocybe cylindracea galectin with lactose
Summary for 3WG1
| Entry DOI | 10.2210/pdb3wg1/pdb |
| Related | 3WG2 3WG3 3WG4 |
| Related PRD ID | PRD_900004 |
| Descriptor | Galactoside-binding lectin, beta-D-galactopyranose-(1-4)-beta-D-glucopyranose (3 entities in total) |
| Functional Keywords | galectin, sugar binding protein |
| Biological source | Agrocybe aegerita |
| Total number of polymer chains | 2 |
| Total formula weight | 38692.80 |
| Authors | Kuwabara, N.,Hu, D.,Tateno, H.,Makio, H.,Hirabayashi, J.,Kato, R. (deposition date: 2013-07-25, release date: 2013-11-27, Last modification date: 2023-11-08) |
| Primary citation | Kuwabara, N.,Hu, D.,Tateno, H.,Makyio, H.,Hirabayashi, J.,Kato, R. Conformational change of a unique sequence in a fungal galectin from Agrocybe cylindracea controls glycan ligand-binding specificity. Febs Lett., 587:3620-3625, 2013 Cited by PubMed Abstract: A fungal galectin from Agrocybe cylindracea (ACG) exhibits broad binding specificity for β-galactose-containing glycans. We determined the crystal structures of wild-type ACG and the N46A mutant, with and without glycan ligands. From these structures and a saccharide-binding analysis of the N46A mutant, we revealed that a conformational change of a unique insertion sequence containing Asn46 provides two binding modes for ACG, and thereby confers broad binding specificity. We propose that the unique sequence provides these two distinct glycan-binding modes by an induced-fit mechanism. PubMed: 24036446DOI: 10.1016/j.febslet.2013.08.046 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.9 Å) |
Structure validation
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