3W8O

Crystal Structure of HasAp with Iron Phthalocyanine

Summary for 3W8O

• Entry DOI: 10.2210/pdb3w8o/pdb
• Related: 3W8M 3WAH
• Descriptor: Heme acquisition protein HasAp, [29H,31H-phthalocyaninato(2-)-kappa~4~N~29~,N~30~,N~31~,N~32~]iron (3 entities in total)
• Functional Keywords: heme binding protein, transport protein
• Biological source: Pseudomonas aeruginosa
• Total number of polymer chains: 2
• Total formula weight: 39228.06

Authors

Shirataki, C.,Shoji, O.,Terada, M.,Sugimoto, H.,Shiro, Y.,Watanabe, Y. (deposition date: 2013-03-20, release date: 2014-01-29, Last modification date: 2023-11-08)

Primary citation

Shirataki, C.,Shoji, O.,Terada, M.,Ozaki, S.,Sugimoto, H.,Shiro, Y.,Watanabe, Y.
Inhibition of Heme Uptake in Pseudomonas aeruginosa by its Hemophore (HasAp ) Bound to Synthetic Metal Complexes
Angew.Chem.Int.Ed.Engl., 53:2862-2866, 2014

PubMed Abstract: The heme acquisition system A protein secreted by Pseudomonas aeruginosa (HasA(p)) can capture several synthetic metal complexes other than heme. The crystal structures of HasA(p) harboring synthetic metal complexes revealed only small perturbation of the overall HasA(p) structure. An inhibitory effect upon heme acquisition by HasA(p) bearing synthetic metal complexes was examined by monitoring the growth of Pseudomonas aeruginosa PAO1. HasA(p) bound to iron-phthalocyanine inhibits heme acquisition in the presence of heme-bound HasA(p) as an iron source.

PubMed: 24604808
DOI: 10.1002/anie.201307889

Experimental method

X-RAY DIFFRACTION (1.85 Å)