3VV4

Crystal structure of cyanobacteriochrome TePixJ GAF domain

Summary for 3VV4

• Entry DOI: 10.2210/pdb3vv4/pdb
• Related: 3W2Z
• Descriptor: Methyl-accepting chemotaxis protein, Phycoviolobilin, green light-absorbing form, SULFATE ION, ... (4 entities in total)
• Functional Keywords: cyanobacteriochrome, phycoviolobilin binding, signaling protein
• Biological source: Thermosynechococcus elongatus
• Total number of polymer chains: 2
• Total formula weight: 46143.84

Authors

Ishizuka, T.,Narikawa, R.,Muraki, N.,Shiba, T.,Kurisu, G.,Ikeuchi, M. (deposition date: 2012-07-14, release date: 2013-01-30, Last modification date: 2024-11-20)

Primary citation

Narikawa, R.,Ishizuka, T.,Muraki, N.,Shiba, T.,Kurisu, G.,Ikeuchi, M.
Structures of cyanobacteriochromes from phototaxis regulators AnPixJ and TePixJ reveal general and specific photoconversion mechanism
Proc.Natl.Acad.Sci.USA, 110:918-923, 2013

PubMed Abstract: Cyanobacteriochromes are cyanobacterial tetrapyrrole-binding photoreceptors that share a bilin-binding GAF domain with photoreceptors of the phytochrome family. Cyanobacteriochromes are divided into many subclasses with distinct spectral properties. Among them, putative phototaxis regulators PixJs of Anabaena sp. PCC 7120 and Thermosynechococcus elongatus BP-1 (denoted as AnPixJ and TePixJ, respectively) are representative of subclasses showing red-green-type and blue/green-type reversible photoconversion, respectively. Here, we determined crystal structures for the AnPixJ GAF domain in its red-absorbing 15Z state (Pr) and the TePixJ GAF domain in its green-absorbing 15E state (Pg). The overall structure of these proteins is similar to each other and also similar to known phytochromes. Critical differences found are as follows: (i) the chromophore of AnPixJ Pr is phycocyanobilin in a C5-Z,syn/C10-Z,syn/C15-Z,anti configuration and that of TePixJ Pg is phycoviolobilin in a C10-Z,syn/C15-E,anti configuration, (ii) a side chain of the key aspartic acid is hydrogen bonded to the tetrapyrrole rings A, B and C in AnPixJ Pr and to the pyrrole ring D in TePixJ Pg, (iii) additional protein-chromophore interactions are provided by subclass-specific residues including tryptophan in AnPixJ and cysteine in TePixJ. Possible structural changes following the photoisomerization of the chromophore between C15-Z and C15-E are discussed based on the X-ray structures at 1.8 and 2.0-Å resolution, respectively, in two distinct configurations.

PubMed: 23256156
DOI: 10.1073/pnas.1212098110

Experimental method

X-RAY DIFFRACTION (2 Å)