3VV1

Crystal Structure of Caenorhabditis elegans galectin LEC-6

Summary for 3VV1

• Entry DOI: 10.2210/pdb3vv1/pdb
• Descriptor: Protein LEC-6, beta-D-galactopyranose-(1-4)-alpha-L-fucopyranose, MAGNESIUM ION, ... (4 entities in total)
• Functional Keywords: galectin, galactose-1, 4-fucose binding, sugar binding protein
• Biological source: Caenorhabditis elegans (nematode)
• Total number of polymer chains: 2
• Total formula weight: 35700.09

Authors

Makyio, H.,Takeuchi, T.,Tamura, M.,Nishiyama, K.,Takahashi, H.,Natsugari, H.,Arata, Y.,Kasai, K.,Yamada, Y.,Wakatsuki, S.,Kato, R. (deposition date: 2012-07-10, release date: 2013-05-22, Last modification date: 2024-03-20)

Primary citation

Makyio, H.,Takeuchi, T.,Tamura, M.,Nishiyama, K.,Takahashi, H.,Natsugari, H.,Arata, Y.,Kasai, K.,Yamada, Y.,Wakatsuki, S.,Kato, R.
Structural basis of preferential binding of fucose-containing saccharide by the Caenorhabditis elegans galectin LEC-6
Glycobiology, 23:797-805, 2013

PubMed Abstract: Galectins are a group of lectins that can bind carbohydrate chains containing β-galactoside units. LEC-6, a member of galectins of Caenorhabditis elegans, binds fucose-containing saccharides. We solved the crystal structure of LEC-6 in complex with galactose-β1,4-fucose (Galβ1-4Fuc) at 1.5 Å resolution. The overall structure of the protein and the identities of the amino-acid residues binding to the disaccharide are similar to those of other galectins. However, further structural analysis and multiple sequence alignment between LEC-6 and other galectins indicate that a glutamic acid residue (Glu67) is important for the preferential binding between LEC-6 and the fucose moiety of the Galβ1-4Fuc unit. Frontal affinity chromatography analysis indicated that the affinities of E67D and E67A mutants for Galβ1-4Fuc are lower than that of wild-type LEC-6. Furthermore, the affinities of Glu67 mutants for an endogenous oligosaccharide, which contains a Galβ1-4Fuc unit, are drastically reduced relative to that of the wild-type protein. We conclude that the Glu67 in the oligosaccharide-binding site assists the recognition of the fucose moiety by LEC-6.

PubMed: 23481096
DOI: 10.1093/glycob/cwt017

Experimental method

X-RAY DIFFRACTION (1.5 Å)