3VF1

Structure of a calcium-dependent 11R-lipoxygenase suggests a mechanism for Ca-regulation

Summary for 3VF1

• Entry DOI: 10.2210/pdb3vf1/pdb
• Related PRD ID: PRD_900003
• Descriptor: 11R-lipoxygenase, beta-D-fructofuranose-(2-1)-alpha-D-glucopyranose, FE (II) ION, ... (4 entities in total)
• Functional Keywords: lox, plat, beta sandwich, c2-like domain, non-heme iron, conformational change, dioxygenase, oxidoreductase
• Biological source: Gersemia fruticosa
• Total number of polymer chains: 2
• Total formula weight: 159079.82

Authors

Eek, P.,Jarving, R.,Jarving, I.,Gilbert, N.C.,Newcomer, M.E.,Samel, N. (deposition date: 2012-01-09, release date: 2012-05-16, Last modification date: 2024-02-28)

Primary citation

Eek, P.,Jarving, R.,Jarving, I.,Gilbert, N.C.,Newcomer, M.E.,Samel, N.
Structure of a Calcium-dependent 11R-Lipoxygenase Suggests a Mechanism for Ca2+ Regulation.
J.Biol.Chem., 287:22377-22386, 2012

PubMed Abstract: Lipoxygenases (LOXs) are a key part of several signaling pathways that lead to inflammation and cancer. Yet, the mechanisms of substrate binding and allosteric regulation by the various LOX isoforms remain speculative. Here we report the 2.47-Å resolution crystal structure of the arachidonate 11R-LOX from Gersemia fruticosa, which sheds new light on the mechanism of LOX catalysis. Our crystallographic and mutational studies suggest that the aliphatic tail of the fatty acid is bound in a hydrophobic pocket with two potential entrances. We speculate that LOXs share a common T-shaped substrate channel architecture that gives rise to the varying positional specificities. A general allosteric mechanism is proposed for transmitting the activity-inducing effect of calcium binding from the membrane-targeting PLAT (polycystin-1/lipoxygenase/α-toxin) domain to the active site via a conserved π-cation bridge.

PubMed: 22573333
DOI: 10.1074/jbc.M112.343285

Experimental method

X-RAY DIFFRACTION (2.473 Å)