3UYX

Crystal structures of globular head of 2009 pandemic H1N1 hemagglutinin

Summary for 3UYX

• Entry DOI: 10.2210/pdb3uyx/pdb
• Related: 3AL4 3UYW
• Descriptor: Hemagglutinin, NITRATE ION (3 entities in total)
• Functional Keywords: influenza virus, subunit vaccine, hemagglutinin (ha), viral protein
• Biological source: Influenza A virus
• Total number of polymer chains: 2
• Total formula weight: 48282.06

Authors

Xuan, C.L.,Shi, Y.,Qi, J.X.,Xiao, H.X.,Gao, G.F. (deposition date: 2011-12-06, release date: 2012-10-24, Last modification date: 2024-11-13)

Primary citation

Xuan, C.L.,Shi, Y.,Qi, J.X.,Zhang, W.,Xiao, H.X.,Gao, G.F.
Structural vaccinology: structure-based design of influenza A virus hemagglutinin subtype-specific subunit vaccines
Protein Cell, 2:997-1005, 2011

PubMed Abstract: There is a great need for new vaccine development against influenza A viruses due to the drawbacks of traditional vaccines that are mainly prepared using embryonated eggs. The main component of the current split influenza A virus vaccine is viral hemagglutinin (HA) which induces a strong antibody-mediated immune response. To develop a modern vaccine against influenza A viruses, the current research has been focused on the universal vaccines targeting viral M2, NP and HA proteins. Crystallographic studies have shown that HA forms a trimer embedded on the viral envelope surface, and each monomer consists of a globular head (HA1) and a "rod-like" stalk region (HA2), the latter being more conserved among different HA subtypes and being the primary target for universal vaccines. In this study, we rationally designed the HA head based on the crystal structure of the 2009-pandemic influenza A (H1N1) virus HA as a model, tested its immunogenicity in mice, solved its crystal structure and further examined its immunological characteristics. The results show that the HA globular head can be easily prepared by in vitro refolding in an E. coli expression system, which maintains its intact structure and allows for the stimulation of a strong immune response. Together with recent reports on some similar HA globular head preparations we conclude that structure-based rational design of the HA globular head can be used for subtype-specific vaccines against influenza viruses.

PubMed: 22231357
DOI: 10.1007/s13238-011-1134-y

Experimental method

X-RAY DIFFRACTION (1.8 Å)