3SDU

Structure of a three-domain sesquiterpene synthase: a prospective target for advanced biofuels production

Summary for 3SDU

• Entry DOI: 10.2210/pdb3sdu/pdb
• Related: 3SAE 3SDQ 3SDR 3SDT 3SDV
• Descriptor: Alpha-bisabolene synthase, CHLORIDE ION, MAGNESIUM ION, ... (5 entities in total)
• Functional Keywords: lyase, terpene synthase
• Biological source: Abies grandis (grand fir,lowland fir,lowland white fir,silver fir,white fir,yellow fir)
• Cellular location: Cytoplasm (Probable): O81086
• Total number of polymer chains: 1
• Total formula weight: 94293.78

Authors

McAndrew, R.P.,Peralta-Yahya, P.P.,DeGiovanni, A.,Pereira, J.H.,Hadi, M.Z.,Keasling, J.D.,Adams, P.D. (deposition date: 2011-06-09, release date: 2011-12-14, Last modification date: 2024-02-28)

Primary citation

McAndrew, R.P.,Peralta-Yahya, P.P.,DeGiovanni, A.,Pereira, J.H.,Hadi, M.Z.,Keasling, J.D.,Adams, P.D.
Structure of a three-domain sesquiterpene synthase: a prospective target for advanced biofuels production.
Structure, 19:1876-1884, 2011

PubMed Abstract: The sesquiterpene bisabolene was recently identified as a biosynthetic precursor to bisabolane, an advanced biofuel with physicochemical properties similar to those of D2 diesel. High-titer microbial bisabolene production was achieved using Abies grandis α-bisabolene synthase (AgBIS). Here, we report the structure of AgBIS, a three-domain plant sesquiterpene synthase, crystallized in its apo form and bound to five different inhibitors. Structural and biochemical characterization of the AgBIS terpene synthase Class I active site leads us to propose a catalytic mechanism for the cyclization of farnesyl diphosphate into bisabolene via a bisabolyl cation intermediate. Further, we describe the nonfunctional AgBIS Class II active site whose high similarity to bifunctional diterpene synthases makes it an important link in understanding terpene synthase evolution. Practically, the AgBIS crystal structure is important in future protein engineering efforts to increase the microbial production of bisabolene.

PubMed: 22153510
DOI: 10.1016/j.str.2011.09.013

Experimental method

X-RAY DIFFRACTION (1.89 Å)