3RTO

Acoustically mounted porcine insulin microcrystals yield an X-ray SAD structure

Summary for 3RTO

Entry DOI	10.2210/pdb3rto/pdb
Descriptor	Insulin, ZINC ION, ... (4 entities in total)
Functional Keywords	hormone
Biological source	Sus scrofa (pig) More
Cellular location	Secreted: P01315 P01315
Total number of polymer chains	4
Total formula weight	11706.07
Authors	Soares, A.S.,Engel, M.A.,Stearns, R.,Datwani, S.,Olechno, J.,Ellson, R.,Skinner, J.M.,Allaire, M.,Orville, A.M. (deposition date: 2011-05-03, release date: 2011-05-18, Last modification date: 2024-11-06)
Primary citation	Soares, A.S.,Engel, M.A.,Stearns, R.,Datwani, S.,Olechno, J.,Ellson, R.,Skinner, J.M.,Allaire, M.,Orville, A.M. Acoustically Mounted Microcrystals Yield High-Resolution X-ray Structures. Biochemistry, 50:4399-4401, 2011 Cited by PubMed Abstract: We demonstrate a general strategy for determining structures from showers of microcrystals. It uses acoustic droplet ejection to transfer 2.5 nL droplets from the surface of microcrystal slurries, through the air, onto mounting micromesh pins. Individual microcrystals are located by raster-scanning a several-micrometer X-ray beam across the cryocooled micromeshes. X-ray diffraction data sets merged from several micrometer-sized crystals are used to determine 1.8 Ǻ resolution crystal structures. PubMed: 21542590 DOI: 10.1021/bi200549x PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.8 Å)

Structure validation