3QKD

Crystal structure of Bcl-xL in complex with a Quinazoline sulfonamide inhibitor

Summary for 3QKD

• Entry DOI: 10.2210/pdb3qkd/pdb
• Descriptor: Bcl-2-like protein 1, (R)-N-(7-(4-((4'-chlorobiphenyl-2-yl)methyl)piperazin-1-yl)quinazolin-4-yl)-4-(4-(dimethylamino)-1-(phenylthio)butan-2-ylamino)-3-nitrobenzenesulfonamide, CHLORIDE ION, ... (5 entities in total)
• Functional Keywords: bcl-2 family fold, apoptosis-inhibitor complex, apoptosis/inhibitor
• Biological source: Homo sapiens (human)
• Cellular location: Mitochondrion membrane; Single-pass membrane protein (By similarity): Q07817
• Total number of polymer chains: 2
• Total formula weight: 43412.29

Authors

Czabotar, P.E.,Smith, B.J. (deposition date: 2011-01-31, release date: 2011-04-06, Last modification date: 2023-11-01)

Primary citation

Sleebs, B.E.,Czabotar, P.E.,Fairbrother, W.J.,Fairlie, W.D.,Flygare, J.A.,Huang, D.C.,Kersten, W.J.,Koehler, M.F.,Lessene, G.,Lowes, K.,Parisot, J.P.,Smith, B.J.,Smith, M.L.,Souers, A.J.,Street, I.P.,Yang, H.,Baell, J.B.
Quinazoline sulfonamides as dual binders of the proteins B-cell lymphoma 2 and B-cell lymphoma extra long with potent proapoptotic cell-based activity.
J.Med.Chem., 54:1914-1926, 2011

PubMed Abstract: ABT-737 and ABT-263 are potent inhibitors of the BH3 antiapoptotic proteins, Bcl-x(L) and Bcl-2. This class of putative anticancer agents invariantly contains an acylsulfonamide core. We have designed and synthesized a series of novel quinazoline-based inhibitors of Bcl-2 and Bcl-x(L) that contain a heterocyclic alternative to the acylsulfonamide. These compounds exhibit submicromolar, mechanism-based activity in human small-cell lung carcinoma cell lines in the presence of 10% human serum. This comprises the first successful demonstration of a quinazoline sulfonamide core serving as an effective benzoylsulfonamide bioisostere. Additionally, these novel quinazolines comprise only the second known class of Bcl-2 family protein inhibitors to induce mechanism-based cell death.

PubMed: 21366295
DOI: 10.1021/jm101596e

Experimental method

X-RAY DIFFRACTION (2.02 Å)