3NHH

Crystal structure of Staphylococcal nuclease variant V23E-L36K at cryogenic temperature

Summary for 3NHH

Entry DOI	10.2210/pdb3nhh/pdb
Related	3bdc 3eji
Descriptor	Nuclease A, THYMIDINE-3',5'-DIPHOSPHATE, CALCIUM ION, ... (4 entities in total)
Functional Keywords	staphylococcal nuclease, hyperstable variant, hydrolase, pdtp, salt bridge
Biological source	Staphylococcus aureus
Cellular location	Nuclease A: Secreted. Nuclease B: Membrane: P00644
Total number of polymer chains	1
Total formula weight	16631.74
Authors	Robinson, A.C.,Schlessman, J.L.,Garcia-Moreno, E.B. (deposition date: 2010-06-14, release date: 2010-06-30, Last modification date: 2023-09-06)
Primary citation	Robinson, A.C.,Castaneda, C.A.,Schlessman, J.L.,Garcia-Moreno E, B. Structural and thermodynamic consequences of burial of an artificial ion pair in the hydrophobic interior of a protein. Proc.Natl.Acad.Sci.USA, 111:11685-11690, 2014 Cited by PubMed: 25074910 DOI: 10.1073/pnas.1402900111 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2 Å)

Structure validation