3MCY
Crystal structure of FimH lectin domain bound to biphenyl mannoside meta-methyl ester.
Summary for 3MCY
| Entry DOI | 10.2210/pdb3mcy/pdb |
| Descriptor | FimH, methyl 4'-(alpha-D-mannopyranosyloxy)biphenyl-3-carboxylate, GLYCEROL, ... (6 entities in total) |
| Functional Keywords | lectin, mannoside, immunogobulin fold, carbohydrate binding protein |
| Biological source | Escherichia coli |
| Total number of polymer chains | 4 |
| Total formula weight | 80090.97 |
| Authors | Ford, B.A.,Hultgren, S.J. (deposition date: 2010-03-29, release date: 2010-06-16, Last modification date: 2024-10-30) |
| Primary citation | Han, Z.,Pinkner, J.S.,Ford, B.,Obermann, R.,Nolan, W.,Wildman, S.A.,Hobbs, D.,Ellenberger, T.,Cusumano, C.K.,Hultgren, S.J.,Janetka, J.W. Structure-based drug design and optimization of mannoside bacterial FimH antagonists. J.Med.Chem., 53:4779-4792, 2010 Cited by PubMed Abstract: FimH-mediated cellular adhesion to mannosylated proteins is critical in the ability of uropathogenic E. coli (UPEC) to colonize and invade the bladder epithelium during urinary tract infection. We describe the discovery and optimization of potent small-molecule FimH bacterial adhesion antagonists based on alpha-d-mannose 1-position anomeric glycosides using X-ray structure-guided drug design. Optimized biarylmannosides display low nanomolar binding affinity for FimH in a fluorescence polarization assay and submicromolar cellular activity in a hemagglutination (HA) functional cell assay of bacterial adhesion. X-ray crystallography demonstrates that the biphenyl moiety makes several key interactions with the outer surface of FimH including pi-pi interactions with Tyr-48 and an H-bonding electrostatic interaction with the Arg-98/Glu-50 salt bridge. Dimeric analogues linked through the biaryl ring show an impressive 8-fold increase in potency relative to monomeric matched pairs and represent the most potent FimH antagonists identified to date. The FimH antagonists described herein hold great potential for development as novel therapeutics for the effective treatment of urinary tract infections. PubMed: 20507142DOI: 10.1021/jm100438s PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.9 Å) |
Structure validation
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