3LU0

Molecular model of Escherichia coli core RNA polymerase

Summary for 3LU0

• Entry DOI: 10.2210/pdb3lu0/pdb
• Related: 3LTI
• EMDB information: 5169
• Descriptor: DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... (6 entities in total)
• Functional Keywords: e. coli rna polymerase, dna-directed rna polymerase, nucleotidyltransferase, transcription, transferase
• Biological source: Escherichia coli; More
• Total number of polymer chains: 5
• Total formula weight: 389693.73

Authors

Darst, S.A. (deposition date: 2010-02-16, release date: 2010-09-29, Last modification date: 2024-02-21)

Primary citation

Opalka, N.,Brown, J.,Lane, W.J.,Twist, K.A.,Landick, R.,Asturias, F.J.,Darst, S.A.
Complete structural model of Escherichia coli RNA polymerase from a hybrid approach.
Plos Biol., 8:e1000483-, 2010

PubMed Abstract: The Escherichia coli transcription system is the best characterized from a biochemical and genetic point of view and has served as a model system. Nevertheless, a molecular understanding of the details of E. coli transcription and its regulation, and therefore its full exploitation as a model system, has been hampered by the absence of high-resolution structural information on E. coli RNA polymerase (RNAP). We use a combination of approaches, including high-resolution X-ray crystallography, ab initio structural prediction, homology modeling, and single-particle cryo-electron microscopy, to generate complete atomic models of E. coli core RNAP and an E. coli RNAP ternary elongation complex. The detailed and comprehensive structural descriptions can be used to help interpret previous biochemical and genetic data in a new light and provide a structural framework for designing experiments to understand the function of the E. coli lineage-specific insertions and their role in the E. coli transcription program.

PubMed: 20856905
DOI: 10.1371/journal.pbio.1000483

Experimental method

ELECTRON MICROSCOPY (11.2 Å)