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3J6L

Kinetic and Structural Analysis of Coxsackievirus B3 Receptor Interactions and Formation of the A-particle

3J6L の概要
エントリーDOI10.2210/pdb3j6l/pdb
関連するPDBエントリー3J6M 3J6N 3J6O
EMDBエントリー5927 5928
分子名称Coxsackievirus and adenovirus receptor, SULFATE ION (3 entities in total)
機能のキーワードcoxsackievirus b3, cvb3, car, cell adhesion
由来する生物種Homo sapiens (human)
タンパク質・核酸の鎖数1
化学式量合計14241.10
構造登録者
Organtini, L.J.,Makhov, A.M.,Conway, J.F.,Hafenstein, S.,Carson, S.D. (登録日: 2014-03-19, 公開日: 2014-04-09, 最終更新日: 2024-10-30)
主引用文献Organtini, L.J.,Makhov, A.M.,Conway, J.F.,Hafenstein, S.,Carson, S.D.
Kinetic and structural analysis of coxsackievirus b3 receptor interactions and formation of the a-particle.
J.Virol., 88:5755-5765, 2014
Cited by
PubMed Abstract: The coxsackievirus and adenovirus receptor (CAR) has been identified as the cellular receptor for group B coxsackieviruses, including serotype 3 (CVB3). CAR mediates infection by binding to CVB3 and catalyzing conformational changes in the virus that result in formation of the altered, noninfectious A-particle. Kinetic analyses show that the apparent first-order rate constant for the inactivation of CVB3 by soluble CAR (sCAR) at physiological temperatures varies nonlinearly with sCAR concentration. Cryo-electron microscopy (cryo-EM) reconstruction of the CVB3-CAR complex resulted in a 9.0-Å resolution map that was interpreted with the four available crystal structures of CAR, providing a consensus footprint for the receptor binding site. The analysis of the cryo-EM structure identifies important virus-receptor interactions that are conserved across picornavirus species. These conserved interactions map to variable antigenic sites or structurally conserved regions, suggesting a combination of evolutionary mechanisms for receptor site preservation. The CAR-catalyzed A-particle structure was solved to a 6.6-Å resolution and shows significant rearrangement of internal features and symmetric interactions with the RNA genome.
PubMed: 24623425
DOI: 10.1128/JVI.00299-14
主引用文献が同じPDBエントリー
実験手法
ELECTRON MICROSCOPY (9 Å)
構造検証レポート
Validation report summary of 3j6l
検証レポート(詳細版)ダウンロードをダウンロード

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件を2025-04-02に公開中

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