3CZZ

Crystal structure of Cyanovirin-N domain B mutant

Summary for 3CZZ

• Entry DOI: 10.2210/pdb3czz/pdb
• Related: 1L5B 2EZM 2Z21
• Descriptor: Cyanovirin-N (2 entities in total)
• Functional Keywords: cyanovirin-n, sugar binding protein, hiv-inactivating, gp120, antiviral protein, protein synthesis inhibitor
• Biological source: Nostoc ellipsosporum
• Total number of polymer chains: 2
• Total formula weight: 21387.49

Authors

Matei, E.,Furey, W.,Gronenborn, A.M. (deposition date: 2008-04-30, release date: 2008-08-19, Last modification date: 2024-11-20)

Primary citation

Matei, E.,Furey, W.,Gronenborn, A.M.
Solution and crystal structures of a sugar binding site mutant of cyanovirin-N: no evidence of domain swapping.
Structure, 16:1183-1194, 2008

PubMed Abstract: The cyanobacterial lectin Cyanovirin-N (CV-N) exhibits antiviral activity against HIV at a low nanomolar concentration by interacting with high-mannose oligosaccharides on the virus surface envelope glycoprotein gp120. Atomic structures of wild-type CV-N revealed a monomer in solution and a domain-swapped dimer in the crystal, with the monomer comprising two independent carbohydrate binding sites that individually bind with micromolar affinity to di- and trimannoses. In the mutant CVN(mutDB), the binding site on domain B was abolished and the protein was found to be completely inactive against HIV. We determined the solution NMR and crystal structures of this variant and characterized its sugar binding properties. In solution and the crystal, CVN(mutDB) is a monomer and no domain-swapping was observed. The protein binds to Man-3 and Man-9 with similar dissociation constants ( approximately 4 muM). This confirms that the nanomolar activity of wild-type CV-N is related to the multisite nature of the protein carbohydrate interaction.

PubMed: 18682220
DOI: 10.1016/j.str.2008.05.011

Experimental method

X-RAY DIFFRACTION (1.36 Å)