376D

A ZIPPER-LIKE DNA DUPLEX D(GCGAAAGCT)

376D の概要

• エントリーDOI: 10.2210/pdb376d/pdb
• 分子名称: DNA (5'-D(*GP*(CBR)P*GP*AP*AP*AP*GP*CP*T)-3'), COBALT HEXAMMINE(III) (3 entities in total)
• 機能のキーワード: right handed dna, double helix, overhanging base, modified, mismatched, dna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 3004.85

構造登録者

Cruse, W.B.T.,Shepard, W.,Prange, T.,delalFortelle, E.,Fourme, R. (登録日: 1998-01-22, 公開日: 1999-10-26, 最終更新日: 2024-04-03)

主引用文献

Shepard, W.,Cruse, W.B.,Fourme, R.,de la Fortelle, E.,Prange, T.
A zipper-like duplex in DNA: the crystal structure of d(GCGAAAGCT) at 2.1 A resolution.
Structure, 6:849-861, 1998

PubMed Abstract: The replication origin of the single-stranded (ss)DNA bacteriophage G4 has been proposed to fold into a hairpin loop containing the sequence GCGAAAGC. This sequence comprises a purine-rich motif (GAAA), which also occurs in conserved repetitive sequences of centromeric DNA. ssDNA analogues of these sequences often show exceptional stability which is associated with hairpin loops or unusual duplexes, and may be important in DNA replication and centromere function. Nuclear magnetic resonance (NMR) studies indicate that the GCGAAAGC sequence forms a hairpin loop in solution, while centromere-like repeats dimerise into unusual duplexes. The factors stabilising these unusual secondary structure elements in ssDNA, however, are poorly understood.

PubMed: 9687367
DOI: 10.1016/S0969-2126(98)00087-2

実験手法

X-RAY DIFFRACTION (2.1 Å)