354D

Structure of loop E FROM E. coli 5S RRNA

354D の概要

• エントリーDOI: 10.2210/pdb354d/pdb
• 分子名称: RNA (5'-R(*CP*CP*GP*AP*UP*GP*GP*UP*AP*GP*UP*G)-3'), RNA-DNA (5'-R(*GP*CP*GP*AP*GP*AP*GP*UP*AP*)-D(*DGP(S)*)-R(*GP*C)-3'), MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: u-rna, double helix, internal loop, overhanging base, mismatched, rna
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 7920.36

構造登録者

Correll, C.C.,Freeborn, B.,Moore, P.B.,Steitz, T.A. (登録日: 1997-10-01, 公開日: 1997-11-26, 最終更新日: 2024-02-21)

主引用文献

Correll, C.C.,Freeborn, B.,Moore, P.B.,Steitz, T.A.
Metals, motifs, and recognition in the crystal structure of a 5S rRNA domain.
Cell(Cambridge,Mass.), 91:705-712, 1997

PubMed Abstract: Two new RNA structures portray how non-Watson-Crick base pairs and metal ions can produce a unique RNA shape suitable for recognition by proteins. The crystal structures of a 62 nt domain of E. coli 5S ribosomal RNA and a duplex dodecamer encompassing an internal loop E have been determined at 3.0 and 1.5 A, respectively. This loop E region is distorted by three "cross-strand purine stacks" and three novel, water-mediated noncanonical base pairs and stabilized by a four metal ion zipper. These features give its minor groove a unique hydrogen-bonding surface and make the adjacent major groove wide enough to permit recognition by the ribosomal protein L25, which is expected to bind to this surface.

PubMed: 9393863
DOI: 10.1016/S0092-8674(00)80457-2

実験手法

X-RAY DIFFRACTION (1.5 Å)