353D

CRYSTAL STRUCTURE OF DOMAIN A OF THERMUS FLAVUS 5S RRNA AND THE CONTRIBUTION OF WATER MOLECULES TO ITS STRUCTURE

Summary for 353D

• Entry DOI: 10.2210/pdb353d/pdb
• Descriptor: RNA (5'-R(*AP*UP*CP*CP*CP*CP*CP*GP*UP*GP*CP*C)-3'), RNA (5'-R(*GP*GP*UP*GP*CP*GP*GP*GP*GP*GP*AP*U)-3') (3 entities in total)
• Functional Keywords: a-rna, double strand, rna
• Total number of polymer chains: 2
• Total formula weight: 7686.67

Authors

Betzel, C.,Lorenz, S.,Furste, J.P.,Bald, R.,Zhang, M.,Schneider, T.R.,Wilson, K.S.,Erdmann, V.A. (deposition date: 1997-09-29, release date: 1997-11-10, Last modification date: 2023-08-02)

Primary citation

Betzel, C.,Lorenz, S.,Furste, J.P.,Bald, R.,Zhang, M.,Schneider, T.R.,Wilson, K.S.,Erdmann, V.A.
Crystal structure of domain A of Thermus flavus 5S rRNA and the contribution of water molecules to its structure.
FEBS Lett., 351:159-164, 1994

PubMed Abstract: This is the first high resolution crystal structure of an RNA molecule made by solid phase chemical synthesis and representing a natural RNA. The structure of the domain A of Thermus flavus ribosomal 5S RNA is refined to R = 18% at 2.4 A including 159 solvent molecules. Most of the 2'-hydroxyl groups as well as the phosphate oxygens are involved either in specific hydrogen bonds in intermolecular contacts or to solvent molecules. The two U-G and G-U base-pairs are stabilized by H-bonds supplied via three water molecules to compensate for the lack of base-pair hydrogen bonds. The structure shows for the first time in detail the importance of highly ordered internal water in stabilizing an RNA structure.

PubMed: 8082756
DOI: 10.1016/0014-5793(94)00834-5

Experimental method

X-RAY DIFFRACTION (2.4 Å)