353D

CRYSTAL STRUCTURE OF DOMAIN A OF THERMUS FLAVUS 5S RRNA AND THE CONTRIBUTION OF WATER MOLECULES TO ITS STRUCTURE

353D の概要

• エントリーDOI: 10.2210/pdb353d/pdb
• 分子名称: RNA (5'-R(*AP*UP*CP*CP*CP*CP*CP*GP*UP*GP*CP*C)-3'), RNA (5'-R(*GP*GP*UP*GP*CP*GP*GP*GP*GP*GP*AP*U)-3') (3 entities in total)
• 機能のキーワード: a-rna, double strand, rna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 7686.67

構造登録者

Betzel, C.,Lorenz, S.,Furste, J.P.,Bald, R.,Zhang, M.,Schneider, T.R.,Wilson, K.S.,Erdmann, V.A. (登録日: 1997-09-29, 公開日: 1997-11-10, 最終更新日: 2023-08-02)

主引用文献

Betzel, C.,Lorenz, S.,Furste, J.P.,Bald, R.,Zhang, M.,Schneider, T.R.,Wilson, K.S.,Erdmann, V.A.
Crystal structure of domain A of Thermus flavus 5S rRNA and the contribution of water molecules to its structure.
FEBS Lett., 351:159-164, 1994

PubMed Abstract: This is the first high resolution crystal structure of an RNA molecule made by solid phase chemical synthesis and representing a natural RNA. The structure of the domain A of Thermus flavus ribosomal 5S RNA is refined to R = 18% at 2.4 A including 159 solvent molecules. Most of the 2'-hydroxyl groups as well as the phosphate oxygens are involved either in specific hydrogen bonds in intermolecular contacts or to solvent molecules. The two U-G and G-U base-pairs are stabilized by H-bonds supplied via three water molecules to compensate for the lack of base-pair hydrogen bonds. The structure shows for the first time in detail the importance of highly ordered internal water in stabilizing an RNA structure.

PubMed: 8082756
DOI: 10.1016/0014-5793(94)00834-5

実験手法

X-RAY DIFFRACTION (2.4 Å)