310D

Crystal structure of 2'-O-Me(CGCGCG)2: an RNA duplex at 1.3 A resolution. Hydration pattern of 2'-O-methylated RNA

310D の概要

• エントリーDOI: 10.2210/pdb310d/pdb
• 分子名称: RNA (5'-R(*(OMC)P*(OMG)P*(OMC)P*(OMG)P*(OMC)P*(OMG))-3'), MAGNESIUM ION (3 entities in total)
• 機能のキーワード: a-rna, double helix, modified, rna
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 4029.33

構造登録者

Adamiak, D.A.,Milecki, J.,Adamiak, R.W.,Dauter, Z.,Rypniewski, W.R. (登録日: 1996-05-25, 公開日: 1997-06-24, 最終更新日: 2024-04-03)

主引用文献

Adamiak, D.A.,Milecki, J.,Popenda, M.,Adamiak, R.W.,Dauter, Z.,Rypniewski, W.R.
Crystal structure of 2'-O-Me(CGCGCG)2, an RNA duplex at 1.30 A resolution. Hydration pattern of 2'-O-methylated RNA.
Nucleic Acids Res., 25:4599-4607, 1997

PubMed Abstract: The molecular and crystal structure of 2'-O-Me (CGCGCG)2 has been determined using synchrotron radiation at near-atomic resolution (1.30 A), the highest resolution to date in the RNA field. The crystal structure is a half-turn A-type helix with some helical parameters deviating from canonical A-RNA, such as low base pair rise, elevated helical twist and inclination angles. In CG steps, inter-strand guanines are parallel while cytosines are not parallel. In steps GC this motif is reversed. This type of regularity is not seen in other RNA crystal structures. The structure includes 44 water molecules and two hydrated Mg2+ions one of which lies exactly on the crystallographic 2-fold axis. There are distinct patterns of hydration in the major and the minor grooves. The major groove is stabilised by water clusters consisting of fused five- and six-membered rings. Minor groove contains only a single row of water molecules; each water bridges either two self-parallel cytosines or two self-parallel guanines by a pair of hydrogen bonds. The structure provides the first view of the hydration scheme of 2'-O-methylated RNA duplex.

PubMed: 9358171
DOI: 10.1093/nar/25.22.4599

実験手法

X-RAY DIFFRACTION (1.3 Å)