2ZZJ

Crystal structure of endo-beta-1,4-glucuronan lyase from fungus Trichoderma reesei

2ZZJ の概要

• エントリーDOI: 10.2210/pdb2zzj/pdb
• 分子名称: Glucuronan lyase A, CALCIUM ION, CITRIC ACID, ... (4 entities in total)
• 機能のキーワード: beta-jelly roll, lyase
• 由来する生物種: Trichoderma reesei (Hypocrea jecorina)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 27569.02

構造登録者

Konno, N.,Ishida, T.,Fushinobu, S.,Igarashi, K.,Habu, N.,Samejima, M.,Isogai, A. (登録日: 2009-02-16, 公開日: 2009-05-05, 最終更新日: 2024-11-13)

主引用文献

Konno, N.,Ishida, T.,Igarashi, K.,Fushinobu, S.,Habu, N.,Samejima, M.,Isogai, A.
Crystal structure of polysaccharide lyase family 20 endo-beta-1,4-glucuronan lyase from the filamentous fungus Trichoderma reesei.
Febs Lett., 583:1323-1326, 2009

PubMed Abstract: The crystal structure of endo-beta-(1-->4)-glucuronan lyase from Trichoderma reesei (TrGL) has been determined at 1.8A resolution as the first three-dimensional structure of polysaccharide lyase (PL) family 20. TrGL has a typical beta-jelly roll fold, which is similar to glycoside hydrolase family 16 and PL7 enzymes. A calcium ion is bound to the site far from the cleft and appears to contribute to the stability. There are several completely conserved residues in the cleft. Possible catalytic residues are predicted based on structural comparison with PL7 alginate lyase A1-II'.

PubMed: 19306878
DOI: 10.1016/j.febslet.2009.03.034

実験手法

X-RAY DIFFRACTION (1.8 Å)