2ZWN

Crystal structure of the novel two-domain type laccase from a metagenome

2ZWN の概要

• エントリーDOI: 10.2210/pdb2zwn/pdb
• 分子名称: two-domain type laccase, COPPER (II) ION, CU-O-CU LINKAGE, ... (5 entities in total)
• 機能のキーワード: laccase, muticopper oxidase, oxidoreductase
• 由来する生物種: metagenomes
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 115430.69

構造登録者

Komori, H.,Miyazaki, K.,Higuchi, Y. (登録日: 2008-12-17, 公開日: 2009-04-07, 最終更新日: 2024-03-13)

主引用文献

Komori, H.,Miyazaki, K.,Higuchi, Y.
X-ray structure of a two-domain type laccase: a missing link in the evolution of multi-copper proteins
Febs Lett., 583:1189-1195, 2009

PubMed Abstract: A multi-copper protein with two cupredoxin-like domains was identified from our in-house metagenomic database. The recombinant protein, mgLAC, contained four copper ions/subunits, oxidized various phenolic and non-phenolic substrates, and had spectroscopic properties similar to common laccases. X-ray structure analysis revealed a homotrimeric architecture for this enzyme, which resembles nitrite reductase (NIR). However, a difference in copper coordination was found at the domain interface. mgLAC contains a T2/T3 tri-nuclear copper cluster at this site, whereas a mononuclear T2 copper occupies this position in NIR. The trimer is thus an essential part of the architecture of two-domain multi-copper proteins, and mgLAC may be an evolutionary precursor of NIR.

PubMed: 19285076
DOI: 10.1016/j.febslet.2009.03.008

実験手法

X-RAY DIFFRACTION (1.7 Å)