2ZVU

Crystal structure of rat heme oxygenase-1 in complex with ferrous verdoheme

2ZVU の概要

• エントリーDOI: 10.2210/pdb2zvu/pdb
• 関連するPDBエントリー: 1DVE 1J2C 1TWN
• 分子名称: Heme oxygenase 1, 5-OXA-PROTOPORPHYRIN IX CONTAINING FE, FORMIC ACID, ... (4 entities in total)
• 機能のキーワード: reaction intermediate bound structure, oxidoreductase, endoplasmic reticulum, heme, iron, metal-binding, microsome, phosphoprotein
• 由来する生物種: Rattus norvegicus (Rat)
• 細胞内の位置: Microsome: P06762
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 31277.99

構造登録者

Sato, H.,Sugishima, M.,Fukuyama, K.,Noguchi, M. (登録日: 2008-11-21, 公開日: 2009-02-03, 最終更新日: 2023-11-01)

主引用文献

Sato, H.,Sugishima, M.,Sakamoto, H.,Higashimoto, Y.,Shimokawa, C.,Fukuyama, K.,Palmer, G.,Noguchi, M.
Crystal structure of rat haem oxygenase-1 in complex with ferrous verdohaem: presence of a hydrogen-bond network on the distal side
Biochem.J., 419:339-345, 2009

PubMed Abstract: HO (haem oxygenase) catalyses the degradation of haem to biliverdin, CO and ferrous iron via three successive oxygenation reactions, i.e. haem to alpha-hydroxyhaem, alpha-hydroxyhaem to alpha-verdohaem and alpha-verdohaem to ferric biliverdin-iron chelate. In the present study, we determined the crystal structure of ferrous alpha-verdohaem-rat HO-1 complex at 2.2 A (1 A=0.1 nm) resolution. The overall structure of the verdohaem complex was similar to that of the haem complex. Water or OH- was co-ordinated to the verdohaem iron as a distal ligand. A hydrogen-bond network consisting of water molecules and several amino acid residues was observed at the distal side of verdohaem. Such a hydrogen-bond network was conserved in the structures of rat HO-1 complexes with haem and with the ferric biliverdin-iron chelate. This hydrogen-bond network may act as a proton donor to form an activated oxygen intermediate, probably a ferric hydroperoxide species, in the degradation of alpha-verdohaem to ferric biliverdin-iron chelate similar to that seen in the first oxygenation step.

PubMed: 19154182
DOI: 10.1042/BJ20082279

実験手法

X-RAY DIFFRACTION (2.2 Å)