2ZV6

Crystal structure of human squamous cell carcinoma antigen 1

Summary for 2ZV6

• Entry DOI: 10.2210/pdb2zv6/pdb
• Descriptor: Serpin B3 (2 entities in total)
• Functional Keywords: serine proteinase inhibitor, reactive site loop, acetylation, alternative splicing, cytoplasm, polymorphism, protease inhibitor, serine protease inhibitor, hydrolase inhibitor
• Biological source: Homo sapiens (Human)
• Cellular location: Cytoplasm: P29508
• Total number of polymer chains: 3
• Total formula weight: 137684.36

Authors

Zheng, B.,Matoba, Y.,Katagiri, C.,Hibino, T.,Sugiyama, M. (deposition date: 2008-11-01, release date: 2009-02-24, Last modification date: 2023-11-01)

Primary citation

Zheng, B.,Matoba, Y.,Kumagai, T.,Katagiri, C.,Hibino, T.,Sugiyama, M.
Crystal structure of SCCA1 and insight about the interaction with JNK1
Biochem.Biophys.Res.Commun., 380:143-147, 2009

PubMed Abstract: Squamous cell carcinoma antigen 1 (SCCA1), which belongs to serine proteinase inhibitor (serpin) superfamily, inhibits papain-like cysteine proteinase. Recently, it has been reported that SCCA1 acts not only as a proteinase inhibitor but also as an inhibitor of UV-induced apoptosis via suppression of the activity of c-Jun NH(2)-terminal kinase (JNK1). The present study determined the crystal structure of SCCA1, suggesting that the reactive center loop (RCL) of SCCA1, a recognition site of proteinase, is very flexible and located away form the main-body of SCCA1. We show that the inhibitory effect of SCCA1 on the kinase activity of JNK1 is lost when the RCL was truncated. Furthermore, we found that a mutant protein created by replacing one amino acid in RCL maintain the suppressive activity to JNK1, whereas the inhibitory effect to proteinase is obviously decreased.

PubMed: 19166818
DOI: 10.1016/j.bbrc.2009.01.057

Experimental method

X-RAY DIFFRACTION (2.7 Å)