2ZTT

Crystal Structure of RNA polymerase PB1-PB2 subunits from Influenza A Virus

2ZTT の概要

• エントリーDOI: 10.2210/pdb2ztt/pdb
• 分子名称: RNA-directed RNA polymerase catalytic subunit, Polymerase basic protein 2 (3 entities in total)
• 機能のキーワード: influenza virus, rna polymerase, pb1-pb2 complex form, nucleotide-binding, nucleotidyltransferase, nucleus, rna replication, rna-directed rna polymerase, transferase, mitochondrion, mrna capping, mrna processing, virion
• 由来する生物種: Influenza A virus (A/Puerto Rico/8/34(H1N1)); 詳細
• 細胞内の位置: Host nucleus (Potential): P03431; Virion: P03428
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 28638.98

構造登録者

Sugiyama, K.,Obayashi, E.,Park, S.-Y. (登録日: 2008-10-08, 公開日: 2009-06-09, 最終更新日: 2024-11-13)

主引用文献

Sugiyama, K.,Obayashi, E.,Kawaguchi, A.,Suzuki, Y.,Tame, J.R.H.,Nagata, K.,Park, S.-Y.
Structural insight into the essential PB1-PB2 subunit contact of the influenza virus RNA polymerase
Embo J., 28:1803-1811, 2009

PubMed Abstract: Influenza virus RNA-dependent RNA polymerase is a multi-functional heterotrimer, which uses a 'cap-snatching' mechanism to produce viral mRNA. Host cell mRNA is cleaved to yield a cap-bearing oligonucleotide, which can be extended using viral genomic RNA as a template. The cap-binding and endonuclease activities are only activated once viral genomic RNA is bound. This requires signalling from the RNA-binding PB1 subunit to the cap-binding PB2 subunit, and the interface between these two subunits is essential for the polymerase activity. We have defined this interaction surface by protein crystallography and tested the effects of mutating contact residues on the function of the holo-enzyme. This novel interface is surprisingly small, yet, it has a crucial function in regulating the 250 kDa polymerase complex and is completely conserved among avian and human influenza viruses.

PubMed: 19461581
DOI: 10.1038/emboj.2009.138

実験手法

X-RAY DIFFRACTION (2.1 Å)