2ZOI

Neutron Crystal Structure of Photoactive Yellow Protein, Wild type, at 295K

2ZOI の概要

• エントリーDOI: 10.2210/pdb2zoi/pdb
• 関連するPDBエントリー: 2ZOH
• 分子名称: Photoactive yellow protein, 4'-HYDROXYCINNAMIC ACID (3 entities in total)
• 機能のキーワード: pas, lov, photoreceptor, light sensor, lbhb, shb, chromophore, photoreceptor protein, receptor, sensory transduction, signaling protein
• 由来する生物種: Halorhodospira halophila
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14052.73

構造登録者

Yamaguchi, S. (登録日: 2008-05-21, 公開日: 2009-03-24, 最終更新日: 2023-11-01)

主引用文献

Yamaguchi, S.,Kamikubo, H.,Kurihara, K.,Kuroki, R.,Niimura, N.,Shimizu, N.,Yamazaki, Y.,Kataoka, M.
Low-barrier hydrogen bond in photoactive yellow protein
Proc.Natl.Acad.Sci.USA, 106:440-444, 2009

PubMed Abstract: Low-barrier hydrogen bonds (LBHBs) have been proposed to play roles in protein functions, including enzymatic catalysis and proton transfer. Transient formation of LBHBs is expected to stabilize specific reaction intermediates. However, based on experimental results and theoretical considerations, arguments against the importance of LBHB in proteins have been raised. The discrepancy is caused by the absence of direct identification of the hydrogen atom position. Here, we show by high-resolution neutron crystallography of photoactive yellow protein (PYP) that a LBHB exists in a protein, even in the ground state. We identified approximately 87% (819/942) of the hydrogen positions in PYP and demonstrated that the hydrogen bond between the chromophore and E46 is a LBHB. This LBHB stabilizes an isolated electric charge buried in the hydrophobic environment of the protein interior. We propose that in the excited state the fast relaxation of the LBHB into a normal hydrogen bond is the trigger for photo-signal propagation to the protein moiety. These results give insights into the novel roles of LBHBs and the mechanism of the formation of LBHBs.

PubMed: 19122140
DOI: 10.1073/pnas.0811882106

実験手法

NEUTRON DIFFRACTION (1.5 Å)