2ZIH

Crystal Structure of Yeast Vps74

2ZIH の概要

• エントリーDOI: 10.2210/pdb2zih/pdb
• 関連するPDBエントリー: 2ZII
• 分子名称: Vacuolar protein sorting-associated protein 74 (2 entities in total)
• 機能のキーワード: beta hairpin, vps, golgi localization, vps74, tetramer, golgi apparatus, phosphoprotein, protein transport, transport
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• 細胞内の位置: Golgi apparatus, Golgi stack membrane ; Peripheral membrane protein ; Cytoplasmic side : Q06385
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 157915.50

構造登録者

Schmitz, K.R.,Li, S.,Setty, T.G.,Ferguson, K.M. (登録日: 2008-02-18, 公開日: 2008-04-22, 最終更新日: 2024-03-13)

主引用文献

Schmitz, K.R.,Liu, J.,Li, S.,Setty, T.G.,Wood, C.S.,Burd, C.G.,Ferguson, K.M.
Golgi localization of glycosyltransferases requires a Vps74p oligomer.
Dev.Cell, 14:523-534, 2008

PubMed Abstract: The mechanism of glycosyltransferase localization to the Golgi apparatus is a long-standing question in secretory cell biology. All Golgi glycosyltransferases are type II membrane proteins with small cytosolic domains that contribute to Golgi localization. To date, no protein has been identified that recognizes the cytosolic domains of Golgi enzymes and contributes to their localization. Here, we report that yeast Vps74p directly binds to the cytosolic domains of cis and medial Golgi mannosyltransferases and that loss of this interaction correlates with loss of Golgi localization of these enzymes. We have solved the X-ray crystal structure of Vps74p and find that it forms a tetramer, which we also observe in solution. Deletion of a critical structural motif disrupts tetramer formation and results in loss of Vps74p localization and function. Vps74p is highly homologous to the human GMx33 Golgi matrix proteins, suggesting a conserved function for these proteins in the Golgi enzyme localization machinery.

PubMed: 18410729
DOI: 10.1016/j.devcel.2008.02.016

実験手法

X-RAY DIFFRACTION (2.8 Å)