2ZI0

Crystal structure of Tav2b/siRNA complex

2ZI0 の概要

• エントリーDOI: 10.2210/pdb2zi0/pdb
• 分子名称: Protein 2b, RNA (5'-D(P*AP*GP*AP*CP*AP*GP*CP*AP*UP*UP*AP*UP*GP*CP*UP*GP*UP*CP*UP*UP*U)-3') (3 entities in total)
• 機能のキーワード: rnai suppression, nucleus, suppressor of rna silencing, gene regulation-rna complex, gene regulation/rna
• 由来する生物種: Tomato aspermy virus (TAV)
• 細胞内の位置: Host nucleus (By similarity): Q8UYT3
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 31585.88

構造登録者

Yuan, Y.A.,Chen, H.-Y. (登録日: 2008-02-12, 公開日: 2008-07-22, 最終更新日: 2024-10-16)

主引用文献

Chen, H.-Y.,Yang, J.,Lin, C.,Yuan, Y.A.
Structural basis for RNA-silencing suppression by Tomato aspermy virus protein 2b
Embo Rep., 9:754-760, 2008

PubMed Abstract: The 2b proteins encoded by cucumovirus act as post-transcriptional gene silencing suppressors to counter host defence during infection. Here we report the crystal structure of Tomato aspermy virus 2b (TAV2b) protein bound to a 19 bp small interfering RNA (siRNA) duplex. TAV2b adopts an all alpha-helix structure and forms a homodimer to measure siRNA duplex in a length-preference mode. TAV2b has a pair of hook-like structures to recognize simultaneously two alpha-helical turns of A-form RNA duplex by fitting its alpha-helix backbone into two adjacent major grooves of siRNA duplex. The conserved pi-stackings between tryptophan and the 5'-terminal base of siRNA duplex from both ends enhance the recognition. TAV2b further oligomerizes to form a dimer of dimers through the conserved leucine-zipper-like motif at its amino-terminal alpha-helix. Biochemical experiments suggest that TAV2b might interfere with the post-transcriptional gene silencing pathway by directly binding to siRNA duplex.

PubMed: 18600235
DOI: 10.1038/embor.2008.118

実験手法

X-RAY DIFFRACTION (2.82 Å)