2Z9W

Crystal structure of pyridoxamine-pyruvate aminotransferase complexed with pyridoxal

2Z9W の概要

• エントリーDOI: 10.2210/pdb2z9w/pdb
• 関連するPDBエントリー: 2Z9U 2Z9V 2Z9X
• 分子名称: Aspartate aminotransferase, SULFATE ION, 3-HYDROXY-5-(HYDROXYMETHYL)-2-METHYLISONICOTINALDEHYDE, ... (5 entities in total)
• 機能のキーワード: aminotransferase, pyridoxamine, pyruvate, pyridoxal, transferase
• 由来する生物種: Mesorhizobium loti
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 84658.45

構造登録者

Yoshikane, Y.,Yokochi, N.,Yamasaki, M.,Mizutani, K.,Ohnishi, K.,Mikami, B.,Hayashi, H.,Yagi, T. (登録日: 2007-09-26, 公開日: 2007-11-06, 最終更新日: 2024-10-30)

主引用文献

Yoshikane, Y.,Yokochi, N.,Yamasaki, M.,Mizutani, K.,Ohnishi, K.,Mikami, B.,Hayashi, H.,Yagi, T.
Crystal structure of pyridoxamine-pyruvate aminotransferase from Mesorhizobium loti MAFF303099
J.Biol.Chem., 283:1120-1127, 2008

PubMed Abstract: Pyridoxamine-pyruvate aminotransferase (PPAT; EC 2.6.1.30) is a pyridoxal 5'-phosphate-independent aminotransferase and catalyzes reversible transamination between pyridoxamine and pyruvate to form pyridoxal and L-alanine. The crystal structure of PPAT from Mesorhizobium loti has been solved in space group P4(3)2(1)2 and was refined to an R factor of 15.6% (R(free) = 20.6%) at 2.0 A resolution. In addition, the structures of PPAT in complexes with pyridoxamine, pyridoxal, and pyridoxyl-L-alanine have been refined to R factors of 15.6, 15.4, and 14.5% (R(free) = 18.6, 18.1, and 18.4%) at 1.7, 1.7, and 2.0 A resolution, respectively. PPAT is a homotetramer and each subunit is composed of a large N-terminal domain, consisting of seven beta-sheets and eight alpha-helices, and a smaller C-terminal domain, consisting of three beta-sheets and four alpha-helices. The substrate pyridoxal is bound through an aldimine linkage to Lys-197 in the active site. The alpha-carboxylate group of the substrate amino/keto acid is hydrogen-bonded to Arg-336 and Arg-345. The structures revealed that the bulky side chain of Glu-68 interfered with the binding of the phosphate moiety of pyridoxal 5'-phosphate and made PPAT specific to pyridoxal. The reaction mechanism of the enzyme is discussed based on the structures and kinetics results.

PubMed: 17989071
DOI: 10.1074/jbc.M708061200

実験手法

X-RAY DIFFRACTION (1.7 Å)