2Z90

Crystal Structure of the Second Dps from Mycobacterium smegmatis

2Z90 の概要

• エントリーDOI: 10.2210/pdb2z90/pdb
• 分子名称: Starvation-inducible DNA-binding protein or fine tangled pili major subunit, FE (II) ION, MAGNESIUM ION, ... (5 entities in total)
• 機能のキーワード: dna-binding protein, quarternary assembly, ferroxidation, dna binding protein
• 由来する生物種: Mycobacterium smegmatis str. MC2 155
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 71762.35

構造登録者

Roy, S.,Saraswathi, R.,Chatterji, D.,Vijayan, M. (登録日: 2007-09-13, 公開日: 2008-04-22, 最終更新日: 2023-11-01)

主引用文献

Roy, S.,Saraswathi, R.,Chatterji, D.,Vijayan, M.
Structural studies on the second Mycobacterium smegmatis Dps: invariant and variable features of structure, assembly and function.
J.Mol.Biol., 375:948-959, 2008

PubMed Abstract: A second DNA binding protein from stationary-phase cells of Mycobacterium smegmatis (MsDps2) has been identified from the bacterial genome. It was cloned, expressed and characterised and its crystal structure was determined. The core dodecameric structure of MsDps2 is the same as that of the Dps from the organism described earlier (MsDps1). However, MsDps2 possesses a long N-terminal tail instead of the C-terminal tail in MsDps1. This tail appears to be involved in DNA binding. It is also intimately involved in stabilizing the dodecamer. Partly on account of this factor, MsDps2 assembles straightway into the dodecamer, while MsDps1 does so on incubation after going through an intermediate trimeric stage. The ferroxidation centre is similar in the two proteins, while the pores leading to it exhibit some difference. The mode of sequestration of DNA in the crystalline array of molecules, as evidenced by the crystal structures, appears to be different in MsDps1 and MsDps2, highlighting the variability in the mode of Dps-DNA complexation. A sequence search led to the identification of 300 Dps molecules in bacteria with known genome sequences. Fifty bacteria contain two or more types of Dps molecules each, while 195 contain only one type. Some bacteria, notably some pathogenic ones, do not contain Dps. A sequence signature for Dps could also be derived from the analysis.

PubMed: 18061613
DOI: 10.1016/j.jmb.2007.10.023

実験手法

X-RAY DIFFRACTION (2.4 Å)