2Z8Z

Crystal structure of a platinum-bound S445C mutant of Pseudomonas sp. MIS38 lipase

Summary for 2Z8Z

• Entry DOI: 10.2210/pdb2z8z/pdb
• Related: 2Z8X
• Descriptor: Lipase, CALCIUM ION, ZINC ION, ... (5 entities in total)
• Functional Keywords: family i.3 lipase, beta-roll, calcium-binding protein, hydrolase
• Biological source: Pseudomonas sp.
• Total number of polymer chains: 1
• Total formula weight: 65346.67

Authors

Angkawidjaja, C.,You, D.J.,Matsumura, H.,Koga, Y.,Takano, K.,Kanaya, S. (deposition date: 2007-09-13, release date: 2007-10-30, Last modification date: 2023-11-01)

Primary citation

Angkawidjaja, C.,You, D.J.,Matsumura, H.,Kuwahara, K.,Koga, Y.,Takano, K.,Kanaya, S.
Crystal structure of a family I.3 lipase from Pseudomonas sp. MIS38 in a closed conformation
FEBS Lett., 581:5060-5064, 2007

PubMed Abstract: The crystal structure of a family I.3 lipase from Pseudomonas sp. MIS38 in a closed conformation was determined at 1.5A resolution. This structure highly resembles that of Serratia marcescens LipA in an open conformation, except for the structures of two lids. Lid1 is anchored by a Ca2+ ion (Ca1) in an open conformation, but lacks this Ca1 site and greatly changes its structure and position in a closed conformation. Lid2 forms a helical hairpin in an open conformation, but does not form it and covers the active site in a closed conformation. Based on these results, we discuss on the lid-opening mechanism.

PubMed: 17923123
DOI: 10.1016/j.febslet.2007.09.048

Experimental method

X-RAY DIFFRACTION (1.8 Å)