2Z8Z

Crystal structure of a platinum-bound S445C mutant of Pseudomonas sp. MIS38 lipase

2Z8Z の概要

• エントリーDOI: 10.2210/pdb2z8z/pdb
• 関連するPDBエントリー: 2Z8X
• 分子名称: Lipase, CALCIUM ION, ZINC ION, ... (5 entities in total)
• 機能のキーワード: family i.3 lipase, beta-roll, calcium-binding protein, hydrolase
• 由来する生物種: Pseudomonas sp.
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 65346.67

構造登録者

Angkawidjaja, C.,You, D.J.,Matsumura, H.,Koga, Y.,Takano, K.,Kanaya, S. (登録日: 2007-09-13, 公開日: 2007-10-30, 最終更新日: 2023-11-01)

主引用文献

Angkawidjaja, C.,You, D.J.,Matsumura, H.,Kuwahara, K.,Koga, Y.,Takano, K.,Kanaya, S.
Crystal structure of a family I.3 lipase from Pseudomonas sp. MIS38 in a closed conformation
FEBS Lett., 581:5060-5064, 2007

PubMed Abstract: The crystal structure of a family I.3 lipase from Pseudomonas sp. MIS38 in a closed conformation was determined at 1.5A resolution. This structure highly resembles that of Serratia marcescens LipA in an open conformation, except for the structures of two lids. Lid1 is anchored by a Ca2+ ion (Ca1) in an open conformation, but lacks this Ca1 site and greatly changes its structure and position in a closed conformation. Lid2 forms a helical hairpin in an open conformation, but does not form it and covers the active site in a closed conformation. Based on these results, we discuss on the lid-opening mechanism.

PubMed: 17923123
DOI: 10.1016/j.febslet.2007.09.048

実験手法

X-RAY DIFFRACTION (1.8 Å)