2Z7A

X-ray crystal structure of RV0760c from Mycobacterium tuberculosis at 2.10 Angstrom resolution

2Z7A の概要

• エントリーDOI: 10.2210/pdb2z7a/pdb
• 関連するPDBエントリー: 2A15 2Z76 2Z77
• 分子名称: Putative steroid isomerase, ACETATE ION (3 entities in total)
• 機能のキーワード: alpha+beta conical barrel, structural genomics, tb structural genomics consortium, tbsgc, isomerase
• 由来する生物種: Mycobacterium tuberculosis
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 61260.20

構造登録者

Cherney, M.M.,Garen, C.R.,James, M.N.G.,TB Structural Genomics Consortium (TBSGC) (登録日: 2007-08-17, 公開日: 2007-09-11, 最終更新日: 2023-08-30)

主引用文献

Cherney, M.M.,Garen, C.R.,James, M.N.
Crystal structure of Mycobacterium tuberculosis Rv0760c at 1.50 A resolution, a structural homolog of Delta(5)-3-ketosteroid isomerase.
Biochim.Biophys.Acta, 1784:1625-1632, 2008

PubMed Abstract: We have determined the X-ray crystal structure of the Mycobacterium tuberculosis (Mtb) gene product encoded by the open reading frame Rv0760c at 1.50 A resolution by single-wavelength anomalous dispersion (SAD) phasing of diffraction data from crystals of the selenomethionine-substituted protein. Refinement against diffraction data from the native protein resulted in R(work)=19.5% and R(free)=21.4%. The X-ray crystal structure shows that the homodimeric Rv0760c polypeptide has an alpha + beta conical barrel fold placing it among many structural neighbors of the nuclear transport factor 2 family (NTF2). This family is highly conserved in terms of structure; however the substrates and individual protein functions are diverse. The structures of native Rv0760c in several different crystal forms and Rv0760c bound to 17beta-estradiol 17-hemisuccinate (EH) have also been solved and analyzed.

PubMed: 18589008
DOI: 10.1016/j.bbapap.2008.05.012

実験手法

X-RAY DIFFRACTION (2.1 Å)