2Z6P

Crystal Structure of the Ufc1, Ufm1 conjugating enzyme 1

2Z6P の概要

• エントリーDOI: 10.2210/pdb2z6p/pdb
• 関連するPDBエントリー: 2Z6O
• 分子名称: Ufm1-conjugating enzyme 1 (2 entities in total)
• 機能のキーワード: ufc1, ufm1, ubiquitin, ubl, polymorphism, ubl conjugation pathway, ligase
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 20038.54

構造登録者

Mizushima , T.,Tatsumi, K.,Ozaki, Y.,Kawakami, T.,Suzuki, A.,Ogasahara, K.,Komatsu, M.,Kominami, E.,Tanaka, K.,Yamane, T. (登録日: 2007-08-06, 公開日: 2007-09-25, 最終更新日: 2011-07-13)

主引用文献

Mizushima, T.,Tatsumi, K.,Ozaki, Y.,Kawakami, T.,Suzuki, A.,Ogasahara, K.,Komatsu, M.,Kominami, E.,Tanaka, K.,Yamane, T.
Crystal structure of Ufc1, the Ufm1-conjugating enzyme
Biochem.Biophys.Res.Commun., 362:1079-1084, 2007

PubMed Abstract: Ubiquitin and ubiquitin-like protein-conjugating enzymes play central roles in posttranslational modification processes. The ubiquitin-fold modifier 1 (Ufm1), one of a variety of ubiquitin-like modifiers, is covalently attached to target proteins via Uba5 and Ufm1-conjugating enzyme 1 (Ufc1), which are analogous to the E1 and E2 ubiquitylation enzymes. As Ufm1-related proteins are conserved in metazoa and plants, the Ufm1 system likely plays important roles in various multicellular organisms. Herein, we report the X-ray structure of human Ufc1 determined at 1.6 A resolution. The Ufc1 structure comprises a canonical E2 domain and an additional N-terminal domain. The Uba5 binding site on Ufc1 was assigned by structural comparison of Ufc1 and Ubc12 and related mutational analyses. In addition, we show that the N-terminal unique domain of Ufc1 contributes to thermal stability.

PubMed: 17825256
DOI: 10.1016/j.bbrc.2007.08.129

実験手法

X-RAY DIFFRACTION (1.8 Å)