2Z4S

Crystal structure of domain III from the Thermotoga maritima replication initiation protein DnaA

2Z4S の概要

• エントリーDOI: 10.2210/pdb2z4s/pdb
• 関連するPDBエントリー: 2Z4R
• 分子名称: Chromosomal replication initiator protein dnaA, MAGNESIUM ION, ADENOSINE-5'-DIPHOSPHATE (3 entities in total)
• 機能のキーワード: aaa+ atpase, domain iii (atpase domain), atp-binding, cytoplasm, dna replication, dna-binding, nucleotide-binding, dna binding protein, structural genomics, nppsfa, national project on protein structural and functional analyses, riken structural genomics/proteomics initiative, rsgi
• 由来する生物種: Thermotoga maritima
• 細胞内の位置: Cytoplasm (By similarity): P46798
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 50832.70

構造登録者

Fujikawa, N.,Ozaki, S.,Kagawa, W.,Park, S.-Y.,Katayama, T.,Kurumizaka, H.,Yokoyama, S.,RIKEN Structural Genomics/Proteomics Initiative (RSGI) (登録日: 2007-06-25, 公開日: 2008-02-19, 最終更新日: 2024-08-14)

主引用文献

Ozaki, S.,Kawakami, H.,Nakamura, K.,Fujikawa, N.,Kagawa, W.,Park, S.-Y.,Yokoyama, S.,Kurumizaka, H.,Katayama, T.
A Common Mechanism for the ATP-DnaA-dependent Formation of Open Complexes at the Replication Origin.
J.Biol.Chem., 283:8351-8362, 2008

PubMed Abstract: Initiation of chromosomal replication and its cell cycle-coordinated regulation bear crucial and fundamental mechanisms in most cellular organisms. Escherichia coli DnaA protein forms a homomultimeric complex with the replication origin (oriC). ATP-DnaA multimers unwind the duplex within the oriC unwinding element (DUE). In this study, structural analyses suggested that several residues exposed in the central pore of the putative structure of DnaA multimers could be important for unwinding. Using mutation analyses, we found that, of these candidate residues, DnaA Val-211 and Arg-245 are prerequisites for initiation in vivo and in vitro. Whereas DnaA V211A and R245A proteins retained normal affinities for ATP/ADP and DNA and activity for the ATP-specific conformational change of the initiation complex in vitro, oriC complexes of these mutant proteins were inactive in DUE unwinding and in binding to the single-stranded DUE. Unlike oriC complexes including ADP-DnaA or the mutant DnaA, ATP-DnaA-oriC complexes specifically bound the upper strand of single-stranded DUE. Specific T-rich sequences within the strand were required for binding. The corresponding conserved residues of the DnaA ortholog in Thermotoga maritima, an ancient eubacterium, were also required for DUE unwinding, consistent with the idea that the mechanism and regulation for DUE unwinding can be evolutionarily conserved. These findings provide novel insights into mechanisms for pore-mediated origin unwinding, ATP/ADP-dependent regulation, and helicase loading of the initiation complex.

PubMed: 18216012
DOI: 10.1074/jbc.M708684200

実験手法

X-RAY DIFFRACTION (3 Å)