2Z3X

Structure of a Protein-DNA Complex Essential for DNA Protection in Spore of Bacillus Species

Summary for 2Z3X

• Entry DOI: 10.2210/pdb2z3x/pdb
• Descriptor: 5'-D(*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*A)-3', 5'-D(*CP*CP*CP*CP*CP*CP*CP*CP*CP*CP*A)-3', Small, acid-soluble spore protein C, ... (4 entities in total)
• Functional Keywords: alpha/beta-type sasp, bacillus subtils spore, protein-dna complex, dna binding protein-dna complex, dna binding protein/dna
• Biological source: Bacillus subtilis; More
• Total number of polymer chains: 5
• Total formula weight: 26741.08

Authors

Lee, K.S.,Jedrzejas, M.J. (deposition date: 2007-06-08, release date: 2008-02-12, Last modification date: 2024-05-29)

Primary citation

Lee, K.S.,Bumbaca, D.,Kosman, J.,Setlow, P.,Jedrzejas, M.J.
Structure of a protein-DNA complex essential for DNA protection in spores of Bacillus species.
Proc. Natl. Acad. Sci. U.S.A., 105:2806-2811, 2008

PubMed Abstract: The DNA-binding alpha/beta-type small acid-soluble proteins (SASPs) are a major factor in the resistance and long-term survival of spores of Bacillus species by protecting spore DNA against damage due to desiccation, heat, toxic chemicals, enzymes, and UV radiation. We now report the crystal structure at 2.1 A resolution of an alpha/beta-type SASP bound to a 10-bp DNA duplex. In the complex, the alpha/beta-type SASP adopt a helix-turn-helix motif, interact with DNA through minor groove contacts, bind to approximately 6 bp of DNA as a dimer, and the DNA is in an A-B type conformation. The structure of the complex provides important insights into the molecular details of both DNA and alpha/beta-type SASP protection in the complex and thus also in spores.

PubMed: 18287075
DOI: 10.1073/pnas.0708244105

Experimental method

X-RAY DIFFRACTION (2.1 Å)