2Z0D

The crystal structure of human Atg4B- LC3(1-120) complex

2Z0D の概要

• エントリーDOI: 10.2210/pdb2z0d/pdb
• 関連するPDBエントリー: 2Z0E
• 分子名称: Cysteine protease ATG4B, Microtubule-associated proteins 1A/1B light chain 3B (3 entities in total)
• 機能のキーワード: papain-like fold, ubiquitin fold, hydrolase-structural protein complex, hydrolase/structural protein
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cytoplasm (Probable): Q9Y4P1; Cytoplasm, cytoskeleton: Q62625
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 54687.35

構造登録者

Satoo, K.,Noda, N.N.,Inagaki, F. (登録日: 2007-05-07, 公開日: 2007-05-22, 最終更新日: 2023-11-01)

主引用文献

Satoo, K.,Noda, N.N.,Kumeta, H.,Fujioka, Y.,Mizushima, N.,Ohsumi, Y.,Inagaki, F.
The structure of Atg4B-LC3 complex reveals the mechanism of LC3 processing and delipidation during autophagy.
Embo J., 28:1341-1350, 2009

PubMed Abstract: Atg8 is conjugated to phosphatidylethanolamine (PE) by ubiquitin-like conjugation reactions. Atg8 has at least two functions in autophagy: membrane biogenesis and target recognition. Regulation of PE conjugation and deconjugation of Atg8 is crucial for these functions in which Atg4 has a critical function by both processing Atg8 precursors and deconjugating Atg8-PE. Here, we report the crystal structures of catalytically inert human Atg4B (HsAtg4B) in complex with processed and unprocessed forms of LC3, a mammalian orthologue of yeast Atg8. On LC3 binding, the regulatory loop and the N-terminal tail of HsAtg4B undergo large conformational changes. The regulatory loop masking the entrance of the active site of free HsAtg4B is lifted by LC3 Phe119, so that a groove is formed along which the LC3 tail enters the active site. At the same time, the N-terminal tail masking the exit of the active site of HsAtg4B in the free form is detached from the enzyme core and a large flat surface is exposed, which might enable the enzyme to access the membrane-bound LC3-PE.

PubMed: 19322194
DOI: 10.1038/emboj.2009.80

実験手法

X-RAY DIFFRACTION (1.9 Å)