2YZC

Crystal structure of uricase from Arthrobacter globiformis in complex with allantoate

Summary for 2YZC

• Entry DOI: 10.2210/pdb2yzc/pdb
• Related: 1UOX 1VAX 1VAY 2YZB 2YZD 2YZE
• Descriptor: Uricase, ALLANTOATE ION (3 entities in total)
• Functional Keywords: uricase, oxidoreductase, allantoate
• Biological source: Arthrobacter globiformis
• Total number of polymer chains: 8
• Total formula weight: 272606.02

Authors

Juan, E.C.M.,Hossain, M.T.,Hoque, M.M.,Suzuki, K.,Sekiguchi, T.,Takenaka, A. (deposition date: 2007-05-05, release date: 2008-05-06, Last modification date: 2025-08-06)

Primary citation

Juan, E.C.,Hoque, M.M.,Shimizu, S.,Hossain, M.T.,Yamamoto, T.,Imamura, S.,Suzuki, K.,Tsunoda, M.,Amano, H.,Sekiguchi, T.,Takenaka, A.
Structures of Arthrobacter globiformis urate oxidase-ligand complexes.
Acta Crystallogr.,Sect.D, D64:815-822, 2008

PubMed Abstract: The enzyme urate oxidase catalyzes the conversion of uric acid to 5-hydroxyisourate, one of the steps in the ureide pathway. Arthrobacter globiformis urate oxidase (AgUOX) was crystallized and structures of crystals soaked in the substrate uric acid, the inhibitor 8-azaxanthin and allantoin have been determined at 1.9-2.2 A resolution. The biological unit is a homotetramer and two homotetramers comprise the asymmetric crystallographic unit. Each subunit contains two T-fold domains of betabetaalphaalphabetabeta topology, which are usually found in purine- and pterin-binding enzymes. The uric acid substrate is bound tightly to the enzyme by interactions with Arg180, Leu222 and Gln223 from one subunit and with Thr67 and Asp68 of the neighbouring subunit in the tetramer. In the other crystal structures, lithium borate, 8-azaxanthin and allantoate are bound to the enzyme in a similar manner as uric acid. Based on these AgUOX structures, the enzymatic reaction mechanism of UOX has been proposed.

PubMed: 18645230
DOI: 10.1107/S0907444908013590

Experimental method

X-RAY DIFFRACTION (1.88 Å)