2YZC

Crystal structure of uricase from Arthrobacter globiformis in complex with allantoate

2YZC の概要

• エントリーDOI: 10.2210/pdb2yzc/pdb
• 関連するPDBエントリー: 1UOX 1VAX 1VAY 2YZB 2YZD 2YZE
• 分子名称: Uricase, ALLANTOATE ION (3 entities in total)
• 機能のキーワード: uricase, oxidoreductase, allantoate
• 由来する生物種: Arthrobacter globiformis
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 272606.02

構造登録者

Juan, E.C.M.,Hossain, M.T.,Hoque, M.M.,Suzuki, K.,Sekiguchi, T.,Takenaka, A. (登録日: 2007-05-05, 公開日: 2008-05-06, 最終更新日: 2025-08-06)

主引用文献

Juan, E.C.,Hoque, M.M.,Shimizu, S.,Hossain, M.T.,Yamamoto, T.,Imamura, S.,Suzuki, K.,Tsunoda, M.,Amano, H.,Sekiguchi, T.,Takenaka, A.
Structures of Arthrobacter globiformis urate oxidase-ligand complexes.
Acta Crystallogr.,Sect.D, D64:815-822, 2008

PubMed Abstract: The enzyme urate oxidase catalyzes the conversion of uric acid to 5-hydroxyisourate, one of the steps in the ureide pathway. Arthrobacter globiformis urate oxidase (AgUOX) was crystallized and structures of crystals soaked in the substrate uric acid, the inhibitor 8-azaxanthin and allantoin have been determined at 1.9-2.2 A resolution. The biological unit is a homotetramer and two homotetramers comprise the asymmetric crystallographic unit. Each subunit contains two T-fold domains of betabetaalphaalphabetabeta topology, which are usually found in purine- and pterin-binding enzymes. The uric acid substrate is bound tightly to the enzyme by interactions with Arg180, Leu222 and Gln223 from one subunit and with Thr67 and Asp68 of the neighbouring subunit in the tetramer. In the other crystal structures, lithium borate, 8-azaxanthin and allantoate are bound to the enzyme in a similar manner as uric acid. Based on these AgUOX structures, the enzymatic reaction mechanism of UOX has been proposed.

PubMed: 18645230
DOI: 10.1107/S0907444908013590

実験手法

X-RAY DIFFRACTION (1.88 Å)