2YT4

Crystal structure of human DGCR8 core

2YT4 の概要

• エントリーDOI: 10.2210/pdb2yt4/pdb
• 分子名称: Protein DGCR8 (2 entities in total)
• 機能のキーワード: dsrbd, rna binding domain, rna binding protein
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 26247.09

構造登録者

Cho, Y.,Sohn, S.Y. (登録日: 2007-04-05, 公開日: 2007-08-21, 最終更新日: 2024-03-13)

主引用文献

Sohn, S.Y.,Bae, W.J.,Kim, J.J.,Yeom, K.H.,Kim, V.N.,Cho, Y.
Crystal structure of human DGCR8 core
Nat.Struct.Mol.Biol., 14:847-853, 2007

PubMed Abstract: A complex of Drosha with DGCR8 (or its homolog Pasha) cleaves primary microRNA (pri-miRNA) substrates into precursor miRNA and initiates the microRNA maturation process. Drosha provides the catalytic site for this cleavage, whereas DGCR8 or Pasha provides a frame for anchoring substrate pri-miRNAs. To clarify the molecular basis underlying recognition of pri-miRNA by DGCR8 and Pasha, we determined the crystal structure of the human DGCR8 core (DGCR8S, residues 493-720). In the structure, the two double-stranded RNA-binding domains (dsRBDs) are arranged with pseudo two-fold symmetry and are tightly packed against the C-terminal helix. The H2 helix in each dsRBD is important for recognition of pri-miRNA substrates. This structure, together with fluorescent resonance energy transfer and mutational analyses, suggests that the DGCR8 core recognizes pri-miRNA in two possible orientations. We propose a model for DGCR8's recognition of pri-miRNA.

PubMed: 17704815
DOI: 10.1038/nsmb1294

実験手法

X-RAY DIFFRACTION (2.6 Å)