2YPG

Haemagglutinin of 1968 Human H3N2 Virus in Complex with Human Receptor Analogue LSTc

2YPG の概要

• エントリーDOI: 10.2210/pdb2ypg/pdb
• 分子名称: HEMAGGLUTININ HA1 CHAIN, CITRATE ANION, HEMAGGLUTININ HA2 CHAIN, ... (11 entities in total)
• 機能のキーワード: viral protein, receptor binding, membrane fusion, influenza virus evolution, glycoprotein
• 由来する生物種: INFLUENZA A VIRUS (A/X-31(H3N2)); 詳細
• 細胞内の位置: Virion membrane; Single-pass type I membrane protein (Potential): P03437 P03437
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 179684.45

構造登録者

Liu, J.,Xiong, X.,Haire, L.F.,Lin, Y.P.,Wharton, S.A.,Martin, S.R.,Coombs, P.J.,Vachieri, S.G.,Christodoulou, E.,Walker, P.A.,Skehel, J.J.,Gamblin, S.J.,Hay, A.J.,Daniels, R.S.,McCauley, J.W. (登録日: 2012-10-30, 公開日: 2012-11-07, 最終更新日: 2024-11-20)

主引用文献

Lin, Y.P.,Xiong, X.,Wharton, S.A.,Martin, S.R.,Coombs, P.J.,Vachieri, S.G.,Christodoulou, E.,Walker, P.A.,Liu, J.,Skehel, J.J.,Gamblin, S.J.,Hay, A.J.,Daniels, R.S.,Mccauley, J.W.
Evolution of the Receptor Binding Properties of the Influenza A(H3N2) Hemagglutinin.
Proc.Natl.Acad.Sci.USA, 109:21474-, 2012

PubMed Abstract: The hemagglutinin (HA) of influenza A(H3N2) virus responsible for the 1968 influenza pandemic derived from an avian virus. On introduction into humans, its receptor binding properties had changed from a preference for avian receptors (α2,3-linked sialic acid) to a preference for human receptors (α2,6-linked sialic acid). By 2001, the avidity of human H3 viruses for avian receptors had declined, and since then the affinity for human receptors has also decreased significantly. These changes in receptor binding, which correlate with increased difficulties in virus propagation in vitro and in antigenic analysis, have been assessed by virus hemagglutination of erythrocytes from different species and quantified by measuring virus binding to receptor analogs using surface biolayer interferometry. Crystal structures of HA-receptor analog complexes formed with HAs from viruses isolated in 2004 and 2005 reveal significant differences in the conformation of the 220-loop of HA1, relative to the 1968 structure, resulting in altered interactions between the HA and the receptor analog that explain the changes in receptor affinity. Site-specific mutagenesis shows the HA1 Asp-225→Asn substitution to be the key determinant of the decreased receptor binding in viruses circulating since 2005. Our results indicate that the evolution of human influenza A(H3N2) viruses since 1968 has produced a virus with a low propensity to bind human receptor analogs, and this loss of avidity correlates with the marked reduction in A(H3N2) virus disease impact in the last 10 y.

PubMed: 23236176
DOI: 10.1073/PNAS.1218841110

実験手法

X-RAY DIFFRACTION (2.85 Å)