2YL8

Inhibition of the pneumococcal virulence factor StrH and molecular insights into N-glycan recognition and hydrolysis

2YL8 の概要

• エントリーDOI: 10.2210/pdb2yl8/pdb
• 関連するPDBエントリー: 2YL5 2YL6 2YL9 2YLA 2YLL
• 分子名称: BETA-N-ACETYLHEXOSAMINIDASE, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose, MANGANESE (II) ION, ... (6 entities in total)
• 機能のキーワード: hydrolase
• 由来する生物種: STREPTOCOCCUS PNEUMONIAE
• 細胞内の位置: Secreted, cell wall; Peptidoglycan-anchor (Potential): P49610
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 52206.28

構造登録者

Pluvinage, B.,Higgins, M.A.,Abbott, D.W.,Robb, C.,Dalia, A.B.,Deng, L.,Weiser, J.N.,Parsons, T.B.,Fairbanks, A.J.,Vocadlo, D.J.,Boraston, A.B. (登録日: 2011-05-31, 公開日: 2011-09-07, 最終更新日: 2024-05-08)

主引用文献

Pluvinage, B.,Higgins, M.A.,Abbott, D.W.,Robb, C.,Dalia, A.B.,Deng, L.,Weiser, J.N.,Parsons, T.B.,Fairbanks, A.J.,Vocadlo, D.J.,Boraston, A.B.
Inhibition of the Pneumococcal Virulence Factor Strh and Molecular Insights Into N-Glycan Recognition and Hydrolysis.
Structure, 19:1603-, 2011

PubMed Abstract: The complete degradation of N-linked glycans by the pathogenic bacterium Streptococcus pneumoniae is facilitated by the large multimodular cell wall-attached exo-β-D-N-acetylglucosaminidase StrH. Structural dissection of this virulence factor using X-ray crystallography showed it to have two structurally related glycoside hydrolase family 20 catalytic domains, which displayed the expected specificity for complex N-glycans terminating in N-acetylglucosamine but exhibited unexpected differences in their preferences for the substructures present in these glycans. The structures of the two catalytic domains in complex with unhydrolyzed substrates, including an N-glycan possessing a bisecting N-acetylglucosamine residue, revealed the specific architectural features in the active sites that confer their differential specificities. Inhibitors of StrH are demonstrated to be effective tools in modulating the interaction of StrH with components of the host, such as the innate immune system. Overall, new structural and functional insight into a carbohydrate-mediated component of the pneumococcus-host interaction is provided.

PubMed: 22078560
DOI: 10.1016/J.STR.2011.08.011

実験手法

X-RAY DIFFRACTION (1.75 Å)