2YKT

Crystal structure of the I-BAR domain of IRSp53 (BAIAP2) in complex with an EHEC derived Tir peptide

2YKT の概要

• エントリーDOI: 10.2210/pdb2ykt/pdb
• 関連するPDBエントリー: 1WDZ 1Y2O
• 分子名称: BRAIN-SPECIFIC ANGIOGENESIS INHIBITOR 1-ASSOCIATED PROTEIN 2, TRANSLOCATED INTIMIN RECEPTOR PROTEIN, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: signaling protein, npy motif, binding pocket
• 由来する生物種: HOMO SAPIENS (HUMAN); 詳細
• 細胞内の位置: Cytoplasm: Q9UQB8
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 30237.31

構造登録者

de Groot, J.C.,Schlueter, K.,Carius, Y.,Quedenau, C.,Vingadassalom, D.,Faix, J.,Weiss, S.M.,Reichelt, J.,Standfuss-Gabisch, C.,Lesser, C.F.,Leong, J.M.,Heinz, D.W.,Buessow, K.,Stradal, T.E.B. (登録日: 2011-05-30, 公開日: 2011-09-07, 最終更新日: 2024-10-23)

主引用文献

De Groot, J.C.,Schluter, K.,Carius, Y.,Quedenau, C.,Vingadassalom, D.,Faix, J.,Weiss, S.M.,Reichelt, J.,Standfuss-Gabisch, C.,Lesser, C.F.,Leong, J.M.,Heinz, D.W.,Bussow, K.,Stradal, T.E.B.
Structural Basis for Complex Formation between Human Irsp53 and the Translocated Intimin Receptor Tir of Enterohemorrhagic E. Coli.
Structure, 19:1294-, 2011

PubMed Abstract: Actin assembly beneath enterohemorrhagic E. coli (EHEC) attached to its host cell is triggered by the intracellular interaction of its translocated effector proteins Tir and EspF(U) with human IRSp53 family proteins and N-WASP. Here, we report the structure of the N-terminal I-BAR domain of IRSp53 in complex with a Tir-derived peptide, in which the homodimeric I-BAR domain binds two Tir molecules aligned in parallel. This arrangement provides a protein scaffold linking the bacterium to the host cell's actin polymerization machinery. The structure uncovers a specific peptide-binding site on the I-BAR surface, conserved between IRSp53 and IRTKS. The Tir Asn-Pro-Tyr (NPY) motif, essential for pedestal formation, is specifically recognized by this binding site. The site was confirmed by mutagenesis and in vivo-binding assays. It is possible that IRSp53 utilizes the NPY-binding site for additional interactions with as yet unknown partners within the host cell.

PubMed: 21893288
DOI: 10.1016/J.STR.2011.06.015

実験手法

X-RAY DIFFRACTION (2.11 Å)